摘要
目的探讨建立一种便捷实用的兔骨关节炎软骨缺损动物模型的方法，以适应软骨组织工程技术修复骨关节炎软骨缺损研究的要求。方法5-7月龄新西兰大白兔22只，雌雄不限，体重2．5～3．0kg。根据侧别，分为改良侧（左侧膝）及对照侧（右侧膝）。改良侧分别切除兔内侧半月板、前十字韧带并在股骨髌沟部制造一直径4mm，深3mm的软骨缺损，对照侧仅切除内侧半月板和前十字韧带。分别在术后第3周和第6周在双侧股骨髁部和髌沟部取材，比较两种骨关节炎动物模型的大体形态及病理变化，进行Mankin评分及统计学分析。结果术后6周，改良侧股骨髌沟软骨缺损仍明显存在，但缺损面直径减小，股骨髌沟墨汁染色均达Ⅳ级，光学显微镜下示股骨髌沟软骨缺损达钙化层以下；而对照侧股骨髌沟墨汁染色均未达Ⅳ级。术后3周，改良侧股骨髌沟部Mankin法OA评分（11．82±1．07）分，对照侧（2．37±0．62）分；术后6周，改良侧股骨髌沟部Mankin法OA评分（13．29±1．15）分，对照侧（5．65±1．03）分；改良侧与对照侧股骨髌沟部Mankin评分比较差异有统计学意义（P〈0．01），但股骨髁部Mankin评分两组比较，差异无统计学意义（P〉0．05）。改良侧关节软骨退变进行性加重。结论改良侧和对照侧均能获得满意的骨关节炎动物模型。改良侧在股骨髌沟处形成一个明显的陈旧性软骨缺损，为应用软骨组织工程技术研究骨关节炎软骨缺损修复提供了一种便捷实用的动物模型。
Objective To establish a simple and pragmatic osteoarthritis model in rabbit knees for the research of cartilage defect repair in osteoarthritis by tissue engineering. Methods Twenty-two New Zealand rabbits （5 to 7 months old, 2.5 to 3.0 kg weight） involved in this study, and all of left knees served as upgrade group, all of right knees served as control group. The anterior cruciate ligaments and medial menisci were resected in the knees of 22 rabbits, and a full-thickness cartilage defects were formed on the femoropatellar grooves of the rabbits in upgrade group. The size of the defect was 4 mm in diameter and 3 mm in depth. Postoperatively, the rabbits were sacrificed at 3 weeks or 6 weeks according to their groups. The femoral condylars and femoropatellar grooves were harvested and studied in both gross morphological, ink-staining and pathohistological aspect. Then Mankin scores and statistical analysis were carried out. Results The degeneration of articular cartilage of the two groups got worse by time. In the femoropatellar grooves of upgrade group, the cartilage defects were present but the areas were diminished at the 6th week after surgery. All of ink-staining were grade IV. But in the control group, none of the femoropatellar grooves in ink-staining were grade IV. The cartilage defects reached cancellated bone according light microscope. Mankin scores of the femoropatellar grooves in upgrade group were significantly higher than those in the control group （P〈 0.01）. There were no significant differences in the femoral condylars scores between the two groups （P 〉0.05）. Conclusion Both methods can develop a satisfactory experimental animal model of osteoarthritis. An obviously old cartilage defect can be obtained at the femoropatellar groove in upgrade group. The novel osteoarthritis model is a simple and pragmatic model and a good option for the research of cartilage tissue engineering.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2007年第10期779-782,共4页
Chinese Journal of Orthopaedics
关键词
骨关节炎
膝
软骨
关节
模型
动物
兔
组织工程
Osteoarthritis, knee
Cartilage, articular
Models, animal
Rabbits
Tissue engineering
