摘要
目的建立小青龙合剂中盐酸麻黄碱的含量测定方法。方法色谱柱为Hypersil ODS柱(250m m×4.6m m,5μm),0.02mol/L磷酸二氢钾溶液(含0.2%三乙胺,用磷酸调节pH值至2.7)-乙腈(97.5∶2.5)为流动相,流速为1.0mL/min,检测波长为210nm。结果盐酸麻黄碱质量浓度在14.16～354μg/m L范围内与峰面积呈良好的线性关系,r=0.9999,平均回收率为98.8%,R SD=1.75%(n=9)。结论该方法简便,结果准确,重现性、专属性好,适用于该制剂的定量分析。
Objective To establish HPLC method for the determination of ephedrine hydrochloride in Xiaoqinglong Mixture. Methods The operating condition were Hypersil ODS （250 mm × 4.6 mm, 5 μm）, 0.02 mol/L potassium dihydrogen phosphoric （containing 0, 2% triethaneamine, the pH was adjusted to 2.7 by phosphoric acid）-acetonitrile（97.5：2.5）,The flow rate was 1.0 mL/min, and the UV detective wavelength was set at 210 nm, Results The linear range of the calibration curve of ephedrine hydrochloride was 14.16-354 μg/mL with a correlation coefficient of 0.999 9. The average recovery rate was 98.8% , RSD was 1.75% （ n = 9 ）. Conclusion The method is simple, accurate and reproducible, It can be used for the determination of ephedrine hydrochloride in Xiaoqinglong Mixture.
出处
《中国药业》
CAS
2007年第20期37-38,共2页
China Pharmaceuticals
