摘要
目的:通过观察创面修复过程中创面愈合率以及碱性成纤维细胞生长因子(basicfibroblast growth factor,bFGF)及其受体(bFGFR)表达,探讨康美肤烧伤膏促进创面愈合的可能机制。方法:建立大鼠Ⅱ度烫伤模型,将动物分为对照组(自然愈合组)和实验组(康美肤烧伤膏组)。采用免疫组化染色半定量分析,测定伤后3、7、14、21天的创面愈合率及创面组织中bFGF和bFGFR的表达。结果:康美肤烧伤膏治疗组创面愈合率高于自然愈合组,两组间差异具有显著性(P<0.05)。康美肤烧伤膏治疗组在创伤愈合的早、中期创面bFGF和bFGFR蛋白的表达明显高于自然愈合组,差异有显著性(P<0.05)。结论:康美肤烧伤膏可能是通过提高创面组织bF-GF和bFGFR的表达,从而促进创面的修复过程。
Objective： To observe the effect of Kangmeifu Burns Ointment（KBO） on expression of basic fibroblastgrowth factor （bFGF） and its receptors（bFGFR） of partial -thickness burn wound in rats,and to discuss its mechanism. Methods： Experimental model of partial - thickness burn wound was established. The rats were randomly divided into two groups, namely, experimental group and control group. The wound surfaces in the experimental group were treated by KBO and those with normal healing as control group. Content of bFGF and its receptors expression in wound surface tissue was also measured by technique of histochemistry at 3 th, 7th, 14th,21 th day postinjury. Results ： The wound healing rate of KBO - treated group was evidently higher than that of the control group （ P 〈 0.05 ）. The total expression of bFGF and bFGFR in experiment groups were signficently higher than that of control groups（ P 〈 0.05）. Conclusion： The promoting effect of arnebia root oils on wound healing may be related to increasing the expression level of bFGF and bFGFR in the skin wound.
出处
《中华中医药学刊》
CAS
2007年第10期2071-2072,共2页
Chinese Archives of Traditional Chinese Medicine
基金
福建省科技厅重点项目(2004I005)
福建省教育厅资助课题(JA04211)
