摘要
目的探讨安宫牛黄注射液对脑外伤后炎性反应因子白细胞介素-1β(IL-1β)和细胞间粘附分子-1(ICAM-1)表达的影响。方法将72只SD大鼠随机分为3组,采Feeney法造成鼠脑挫裂伤模型,对照组和治疗组大鼠分别在伤后6、24、48、72h时间点完整取出脑组织,匀浆后离心,取上清液0.5ml,用酶联免疫吸附实验法(ELISA)做IL-1β因子和ICAM-1水平检测。结果治疗组脑组织IL-1β和ICAM-1水平在伤后6h、24h、48h时间点显著低于对照组(P<0.05)。结论安宫牛黄注射液可抑制脑外伤后脑内IL-1β和ICAM-1的表达,减轻炎性反应,对脑组织起保护作用。
Objective To observe the effect of Angongniuhuang injection on the expression of brain interleukin-lbeta （IL-1β） and intercellular adhesion molecule-1 （ICAM-1） after cerebral trauma of rats. Methods All of 72 SD rats were divided into three groups and were hit by Feeney＇s method. The levels of brain IL一1β and ICAM-1 were measured by enzyme linked immunosorbent assay （ELISA） at different time points after hitting. Results The levels of brain IL-1β and ICAM-1 in treated groups were lower than that in control groups at 6 h, 24 h and 48 h after hitting （P 〈 0. 05 ）. Conclusion Angongniuhuang injection can restrain the expression of brain IL-1β and ICAM-1 and protect the brain by alleviating inflammatory responses.
出处
《中华神经外科疾病研究杂志》
CAS
2007年第5期417-419,共3页
Chinese Journal of Neurosurgical Disease Research
