摘要
目的研究不同剂量重组复合膳食纤维对高脂血症大鼠肠道形态结构的影响。方法健康、断乳SD大鼠60只,雌雄各半,经高脂饲料诱导形成高脂血症模型后,按体重及血胆固醇水平均衡的原则分为5组,其中2组分别以基础饲料及高脂饲料作为阴性和阳性对照组,其余3组为5%,10%,20%不同剂量的重组复合膳食纤维,实验期90d,实验期末,用光镜、电镜形态观察及测量分析的方法观察大鼠肠道形态结构。结果20%剂量水平的重组复合膳食纤维可增加粪重(P<0.05),而5%,10%重组复合膳食纤维却可减轻粪重(P<0.05);与高脂对照组比较,20%剂量水平的重组复合膳食纤维组小肠壁重量显著增加,5%,10%剂量水平组动物小肠壁重量明显减轻,差异均有统计学意义(P<0.05);10%,20%剂量组动物的小肠粘膜肌层增厚(P<0.05),10%剂量组动物小肠绒毛略有变短、变宽,20%剂量组的动物小肠绒毛出现水肿,排列紊乱,线粒体肿胀。结论长期摄入低剂量重组复合膳食纤维对大鼠肠道的形态结构无明显影响,但高剂量可能会对肠道的形态结构产生不利影响。
Objective To investigate the long- term effect of artificial fiber complexes （AFC） on the intestinal structure in hypercholesterolemic rats. Methods 60 healthy SD rats were fed with food rich in lipids and hypercholesterolemic, and hypercholesterolemic rats animal models were established. Male and female weanling SD strain rats（80- 100g） were divided into 5 groups, 12 rats per group. As the negative and positive control groups, rats were fed either the normal diet or high - cholesterol diet respectively. The left groups were given one of the diets incorporated AFC at the different level of 5 %, and 10 % or 20% .The experiment lasted 3 months. At the end of the experiment, morphology of the small intestine were observed by light and electron microscope examination. Results Fecal weight could be raised in 20 % of AFC group, but lowered in 5 %, 10 % of AFC groups （P 〈 0.05） ;the weights of small intestine wall in 20 % AFC was significantly higher than both of control groups, but 5 %, 10 % of AFC could lower small intestine wall weight （ P 〈 0.05）. Except thickened muscle layer of small intestine were observed in 10% and 20% AFC group （P〈 0.05）, the left small intestine structure was same as the normal control group. Widen villi and thickened muscle layer of small intestine were observed in 10% AFC group, microvilla becoming short and/or absent, mitoehondria swelling, impairment of the integrity of the cristae were commonly observed in 20% AFC group. Conclusion Long-term intake of AFC at lower levels could be beneficial for small intestine＇s structure;on the other hand, long - term intake of AFC at higher levels could induce some morphological changes of intestine.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第10期1272-1274,共3页
Chinese Journal of Public Health
基金
国家教委基金(7069601)
山西省归国人员基金(6309243)
关键词
重组复合纤维
肠道形态结构
高脂血症
artificial fiber complexes
intestinal structure
hypercholesterolemi
