摘要
环介导的恒温扩增(LAMP)是近年来新兴的一种快速DNA扩增技术,其基本原理在于利用4个特殊设计的引物和具有链置换活性的DNA聚合酶,在恒温条件下对靶序列进行扩增。LAMP技术具有特异性强、敏感性高、反应迅速、设备简单等特点,在人类病毒性疾病的诊断领域有着广泛的应用。
A novel, rapid and sensitive technique called loop-mediated isothermal amplification（LAMP） has been developed recently. This method employs a DNA polymerase that have activity of strand displacement DNA synthesis and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. The LAMP technology is extremely rapid, cost effective, sensitive and specific and now widely used in virus detection.
出处
《生物技术通讯》
CAS
2007年第5期859-862,共4页
Letters in Biotechnology
关键词
环介导的恒温扩增
核酸
病毒
检测
loop-mediated isothermal amplification
nucleic acid
virus
detection
