摘要
目的:探讨木樨草素对肝癌细胞增殖的抑制效应及可能机制,为其临床应用提供理论依据。方法:以肝癌细胞系SMMC7721为模型,通过细胞增殖检测,乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、碱性磷酸酶(ALP)活性及其胞内总蛋白含量检测等手段,观察木樨草素对肝癌SMMC7721细胞的抑制作用。结果:经50μg/mL木樨草素处理72h后,肝癌SMMC7721细胞的生长抑制率达到54.68%;经400μg/mL木樨草素处理24h后,细胞LDH、SDH和ALP的活性与对照组相比分别降低56.12%、49.69%和76.31%;细胞胞内总蛋白含量无明显变化。结论:木樨草素能有效地抑制肝癌SMMC7721细胞的增殖,具有体外抗肿瘤活性,以及与诱导分化药物相似的抗肿瘤效果。
Objective： To explore the effect of luteolin on proliferation of liver cancer SMMC7721 cell line. Methods： The effect of luteolin on tumor cell proliferation was determined by MTT assay. The activities changes of succinate dehydrogenase, lactate dehydrogenase and alkaline phosphatase were determined by colorimetric assay. Results： The proliferation of SMMC7721 cells was inhibited obviously after treating 72 h with 50 μg/mL luteolin, the growth inhibitory rate amounted to 54.68%. In the meantime, luteolin could decrease the activities of lactate dehydrogenase, succinic dehydrogenase and alkaline phosphatase to 56.12%, 49.59% and 76.31%, respectively. Conclusion： Luteolin has showed an anti-tumor activity in vitro.
出处
《生物技术通讯》
CAS
2007年第5期798-799,共2页
Letters in Biotechnology
关键词
木樨草素
抗肿瘤活性
肝癌
luteolin
anti-tumor activity in vitro
liver cancer
