摘要
以穿梭质粒PYES2转化酿酒酵母H158，用二硫苏糖醇（DTT）和处理酵母细胞，变性单链鱼精DNA（ssDNA）为质粒DNA的携带体；转化率比普通酵母完整细胞转化法提高200倍，可达8．3×104个转化子/μg质粒DNA。
Transformation was performed to transfer shuttle plasmid pYES2 into Saccharomyces.cerevisiae. DTT and ssDNA can highly increase transformation efficiency. Using DTT andssDNA together to transform yeast cells which are harvested at the exponential growthphase, transformation efficiency is up to 8. 3×104 transformants/μg DNA.
出处
《生物技术》
CAS
CSCD
1997年第3期37-38,48,共3页
Biotechnology
