摘要
【目的】采用化学分离分析和生物检测相结合的方法,首次从蝉拟青霉[Paecilomyces cicadae(Miq.)Samson]子实体提取物中分离出对小菜蛾[Plitella xylostella(Lepidoptera:Plutellidae)]具有杀虫活性的化合物。【方法】在活性检测引导下,蝉拟青霉子实体40%乙醇提取物经二氯甲烷、乙酸乙酯、正丁醇溶剂依次萃取(剩余溶液为水溶组分),再经过大孔树脂、HPLC和Sephedex LH-20分离纯化得到杀虫活性化合物。【结果】分离纯化到一个白色粉末状化合物,浓度10mg·ml-1,24、48和72h时校正死亡率分别为(95.1±1.8)%、(97.7±1.6)%和(99.1±1.7)%。【结论】该化合物具有较好的杀小菜蛾活性,对酸稳定,对胃蛋白酶不敏感,并具有茚三酮不显色等其它理化性质。
【Objective】Insecticidal active substance of the fruiting body of Paecilomyces Cicadae（Miquel）Samson that can kill the larvae of Plutella xylostella was isolated and purified by combined separation with biology experiments for the first time.【Method】 By means of bioactive guided isolation of 40% ethanol-H20,dichloromethane,ethyl acetate,n-butyl ethanol,macroporous adsorption resin column,high performance liquid chromatography and sephadex A-20 column,the insecticidal active compound was purified.【Result】 A white powder compound was obtained.The compound was active against the third instar larvae of P.xylostella L.in 24,48 and 72 h at the concentration of 10.0 mg·ml^-1 with the corrective mortality of（95.1±1.8）%,（97.7±1.6）% and（99.1±1.7）%,respectively.【Conclusion】The compound was strongly sensitive to the larvae.It was stable to acid,and was not sensitive to pepsin.Other physical and chemical characteristics were reported as well.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第9期1952-1958,共7页
Scientia Agricultura Sinica
基金
温州市重大项目(N2005A018)
浙江省温州市科技项目(S2003A013)
关键词
蝉拟青霉
子实体提取物
小菜蛾
杀虫活性
Paecilomyces cicadae （Miquel） Samson
Extracts of the fruit body
Plutella xylostella（Lepidoptera：Plutellidae）
Insecticidal activity
