摘要
以从铬矿渣堆放区土壤分离到的土著微生物为试验菌群,采用二苯碳酰二肼显色法,检测Cr(Ⅵ)浓度变化,筛选出4株对Cr(Ⅵ)的清除率大于50%的土著菌株,并设定为联合修复的微生物元素,与玉米幼苗作组合,对Cr进行微生物-植物联合清除试验。结果表明,在Cr浓度为100mg·L-1的条件下,具有明显的降低培养液中Cr(Ⅵ)浓度的效果,实验条件下的清除率可达70%;在Cr浓度为100mg·L-1的条件下,土著微生物的存在,还可以明显提高玉米种子萌发率,提高幅度高出对照10%以上。
Effects of union bioremediation on chromium was assayed by using maize and endemic microbe isolated from chromium polluted soil as the plant and microbial factors separately, and DPCZ reaction was adopted for the determination of Cr （Ⅵ）. Cr （Ⅵ） was, in form of K2Cr2O7, directly added into the Hoagland solution with the maize seedlings. The results showed that, at 100 mg·L^-1 of Cr （Ⅵ）, both endemic microbe and maize had the ability to decrease the concentration of Cr （Ⅵ） at laboratory scale. 4 bacterial strains were identified to be tolerant and accumulative to Cr, and they showed more efficiency when mixed together. The union removal effect of maize and endemic microbe on Cr （Ⅵ） could be over 50% when 10^5 mL^-1 bacteria were added and might be up to 70% when the bacterial concentration was 106 mL^-1. In addition, endemic bacteria could obviously increase the germination rate of the maize seed by about 10% than that of the CK in the presence of 100 mg·L^-1 Cr （Ⅵ）.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2007年第5期1870-1873,共4页
Journal of Agro-Environment Science
基金
天津市教委资助项目(20040813)
