摘要
目的探讨血管紧张素（1-7）对血管紧张素Ⅱ诱导心脏成纤维细胞增殖和胶原合成的影响。方法分离培养SD仔鼠心脏成纤维细胞,四氮唑盐比色法检测细胞增殖,流式细胞分析技术测定细胞周期,逆转录—聚合酶链式反应测定心脏成纤维细胞Ⅰ、Ⅲ型胶原mRNA的表达。结果①1.0μmol/L血管紧张素Ⅱ作用24h,心脏成纤维细胞的四氮唑蓝比色分析法吸光度值（0.24±0.01）较无血清对照组（0.14±0.01）明显增加（P〈0.01）;0.001-1.0μmol/L血管紧张素（1-7）和1.0μmol/L血管紧张素Ⅱ共同干预后吸光度值逐渐降低,分别为0.20±0.01、0.19±0.01、0.13±0.01、0.16±0.03,均显著低于血管紧张素Ⅱ组（P〈0.01）。②血管紧张素Ⅱ组心脏成纤维细胞的S期百分率（14.0%±0.9%）和增殖指数（23.4±1.8）较对照组（5.4%±0.7%和10.8±2.4）明显增高（P〈0.01）;0.1μmol/L血管紧张素（1-7）干预后S期百分率（8.5%±0.7%）和增殖指数（16.2±2.0）较血管紧张素Ⅱ组降低（P〈0.01）。③血管紧张素Ⅱ刺激后心脏成纤维细胞的Ⅰ、Ⅲ型胶原mRNA表达水平分别为较对照组明显增高（P〈0.01）;给予0.001-1.0μmol/L血管紧张素（1-7）和1.0μmol/L血管紧张素Ⅱ共同干预后,胶原表达水平浓度依赖性的下降,均显著低于血管紧张素Ⅱ组（P〈0.05）。结论血管紧张素（1-7）具有抑制血管紧张素Ⅱ浓度增加引起的心脏成纤维细胞增殖和胶原合成的作用。
Aim To investigate the effect of Angiotensin（1-7） （Ang（1-7） on cell proliferation and collagen synthesis of cardiac fibroblasts（CF） induced by Angiotensin Ⅱ （Ang Ⅱ）. Methods CF were isolated and cultured from Neonatal SD rats. Cell number and cell cycle were evaluated by MTT assay and flowcytometry technique. Type and Ⅲ collagen mRNA expressions in CF were analyzed by RT-PCR. Results ①1 μmol/L Ang Ⅱ significantly increased absorption value of CF（0.24±0.01）compared with control group （0.14±0.01, P〈0.01）, 0.001～1.0 μmol/L Ang（1-7） attenuated the absorption value of Ang Ⅱ group in a concentration dependent manner （0.22±0.01, 0.21±0.01, 0.18±0.01 and 0.16±0.01, P〈0.01）. ②Compared to the control （5.4%±0.7%, 10.8±2.4）, cells in S stage （14.0%±0.9%） and proliferatiion index （23.4±1.8）, they were markedly increased in 1 μmol/L Ang Ⅱ group （P〈0.01）. In the presence of 0.1 μmol/L Ang（1-7）, cells in S stage （8.5%±0.7%） and the proliferatiion index （16.2±2.0） were significantly lower than that of Ang Ⅱ group （P〈0.01）. ③mRNA expressions of typeⅠand Ⅲ collagen in Ang Ⅱ group were significantly higher than those of the control （P〈0.01）, and in the presence of 0.1 μmol/L Ang（1-7）, mRNA expressions were reduced in a concentration dependent manner （P〈0.05）. Conclusion The results indicate that Ang（1-7） could inhibi the proliferation of CF and the synthesis of type Ⅰand Ⅲ collagen.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第5期361-364,共4页
Chinese Journal of Arteriosclerosis
