摘要
目的研究线粒体融合素基因(mfn2)在正常体重和肥胖乳腺癌患者的不同组织中的表达情况,探讨mfn2基因与乳腺癌发生的关系及评价肥胖与乳腺癌发病的关系。方法采用RT-PCR技术对20例正常体重乳腺癌患者及20例肥胖乳腺癌患者的胸大肌组织,脂肪组织,正常乳腺组织,癌旁组织和癌组织的mfn2的表达水平进行了测定和对比分析。结果两组乳腺癌患者不同组织的mfn2的表达水平有差异。在正常体重组内,胸大肌组织和正常乳腺组织内的表达无显著性差异(P=0.101),但显著高于脂肪组织(P=0.016),高于癌旁组织(P=0.027),高于癌组织(P=0.002)。肥胖组内,胸大肌组织、脂肪组织、正常乳腺组织内的表达无显著性差异(P>0.05),但显著高于癌旁组织(P=0.003),高于癌组织(P=0.001)。两组间比较,mfn2在肥胖组患者的五种组织中的表达较正常体重组均有显著性降低(P<0.05)。结论mfn2基因在癌组织中低表达,且在肥胖患者组织中表达更低,mfn2与乳腺癌的发生有关且肥胖可能增加患乳腺癌的风险。
Objective To study the expression of mfn2 gene in different tissues of normal and overweight patients with breast cancer, and to investigate the relationships between mfn2, obesity and breast cancer. Methods The expressions of mfn2 were measured and analyzed in skeletal muscle, fat, galactophore, para-cancer tissue and cancer using the RT-PCR technique. Results In patients with normal weight, the expressions of mfn2 in muscle and galactophore were not significantly different （ P = 0. 101 ）, and they were significantly higher than in fat （ P = 0. 016 ）, para-cancer （P = 0. 027 ） and cancer （P = 0. 002） . In the overweight group, the expressions in muscle, fat and galactophore were close to each other, but higher than in para-cancer （P = 0. 003 ） and cancer （P = 0. 001 ） . Compared with those of the normal-weight group, the expressions of mfn2 were remarkably lower in the overweight group. Conclusion The results demonstrate that mfn2 has a decreased expression in cancer, especially in the tissues of overweight subjects. Mfn2 is responsible for breast cancer, and obesity may increase the risk of breast cancer.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2007年第4期407-410,共4页
Chinese Journal of Histochemistry and Cytochemistry
