摘要
通过对生物结皮中土生绿球藻(Chlorococcum humicola)的分离、纯化及培养,初步探讨了pH、光照强度、温度和3种不同浓度的K+,Ca2+,Mg2+等因子对土生绿球藻生长的影响。结果表明:pH和温度对土生绿球藻生长的影响极显著,Ca2+,Mg2+浓度影响显著,K+和光照强度影响不显著。最适pH为12,最适温度是25℃,Ca2+和Mg2+的最适浓度分别为1.77×10-4mol/L和2.04×10-5mol/L。其研究结果将为生物结皮的进一步人工培养奠定理论基础。
Abstract： Chlorococcum humicola is a common species, and it can grow not only in humid soil, but also in arid and semiarid desert habitats. In this paper, the effects of pH, illumination intensity, temperature and three different concentrations of K^＋ , Ca^2＋ and Mg^2＋ on the growth of Chlorococcum humicola in soil mierobiotie crusts are studied ture on by separating, purifying and cultivating C. humicola. The results show that the effects of pH and temperature on the growth of C. humicola are extremely significant, that of Ca^2＋ and Mg^2＋ concentrations on the growth of C. humicola are significant, but that of K^＋ concentration and illumination intensity on the growth of C. humicola are not so significant. The results also reveal that alkaline soil is advantageous for the growth of C. humicola, and the biomass of C. humicola growing under the acidic conditions is ten or several ten times lower than that under the alkaline conditions ; the optimal conditions of cultivating C. humicola are at pH of 12, temperature of 25 ℃, and Ca^2＋ and Mg^2＋ concentrations of 1.77 × 10^-4 mol · L^-1 and 2.04 × 10-s mol · L^-1, respectively. The study resuits can be regarded as the theoretic bases in artificially cultivating soil mierobiotie crusts in the further.
出处
《干旱区研究》
CSCD
北大核心
2007年第5期641-646,共6页
Arid Zone Research
基金
中国科学院知识创新工程重要方向项目(KZCX3-SW-343)
国家自然科学基金(40571085
90202019)资助
