摘要
对不同品种健康桑树植株组织中的内生细菌进行分离,共得到229个菌株。探讨了较合理的内生细菌分离纯化方法,对内生细菌数量进行了测定。结果表明,桑树根、茎、叶柄、叶片、花蕾等组织内均存在大量的内生细菌,且不同品种及组织中内生细菌的数量均存在差异。离体抑菌作用测定表明,229株桑树内生细菌中,有42株(18.3%)菌株对桑树炭疽病菌有拮抗作用,有25株(10.9%)菌株对桑粘格孢菌有拮抗作用;以上67株菌种又有8株菌株对多种病原真菌都有抑菌作用,表现出较强抑菌活性,具有作为生防菌的应用潜能。对8株内生拮抗菌株进行了细菌学鉴定,结果表明,菌株G21、G49、Y12和J26归属于芽孢杆菌属(Bacillussp.),G82和J50归属于假单孢菌属(Pseudom onas sp.),Y33归属于欧文氏菌属(Erwinia sp.),B19归属于短小杆菌属(Curtobacterium sp.)。
229 endophytic bacteria strains were isolated from the tissues of different healthy mulberry varieties. The reasonable method for isolation of endophytic bacteria and purification was discussed, and the amount of endophytic bacteria was determined. The results showed that endophytic bacteria exist in all mulberry tissues including root, stem, leaf stalk, lamina and flower bud. But the population densities vary with varieties, tissues and growth periods of the plant. In vitro antagonistic examination revealed that in all 229 tested strains 42 strains （18. 3% ） showed antibiosis activities against Colletotrichum rnorifolium Hara, 25 strains（ 10. 9% ） showed antibiosis activities against Aecidium mori （Bard） Diet and 8 strains showed strong inhibitory activities against some plant fungal pathogens, revealing potential in the aspect of antagonistic action. Bacteriological analysis showed that these 8 strains were belong to Bacillus sp. （G21, G49, Y12 and J26）, Pseudomonas sp. （G82 and J50）, Erwinia sp. （Y33）and Curtobacterium sp. （B19）, respectively.
出处
《蚕业科学》
CAS
CSCD
北大核心
2007年第3期350-354,共5页
ACTA SERICOLOGICA SINICA
基金
山东省农业良种工程项目(编号2002228)
关键词
桑树
内生细菌
生物防治
菌株分离筛选
Mulberry
Endophytic bacteria
Biological control
Isolation and screening of bacterial strain
