摘要
以携带Cry1Ab基因的"克螟稻"为抗虫供体亲本,将目标基因转导到优良恢复系"R6547"、"R818"中,配制的杂交稻组合表现良好的杂种优势水平,克服了"克螟稻"在常规粳稻育种实践中常出现的农艺性状差、前期生长势弱的缺陷。可溶性蛋白含量检测结果显示目标基因在杂交稻中仍能高水平的表达,并在人工接虫和自然发生条件下对稻纵卷叶螟、二化螟等鳞翅目害虫表现优良抗性。
Selecting CrylAb gene-carrying rice germplasm “Kemingdao” as the insect-resistant donor, we transferred the target gene into two elite rice restorer lines“R6547”and “R818” through crossing and backcrossing. The hybrid combinations derived from two transgenic restorer lines show significant heterosis that do overcome the defects of undesirable agronomic traits and weak prophase development capabilities. Results of testing the CrylAb protein indicate that the target gene expresses strongly and provides good resistance to stripe stem borers（ Chilo suppressalis ） and leaf folders（ Cnapha locrocis medinalis） in hybrid combinations.
出处
《中国生态农业学报》
CAS
CSCD
2007年第5期147-150,共4页
Chinese Journal of Eco-Agriculture
基金
国家"863"项目(2002AA2070002)
江苏省高技术项目(BG2004301)资助
关键词
水稻
CrylAb基因
恢复系
杂种优势
抗虫供体
抗虫性鉴定
Rice, Cry1Ab gene, Restorer line, Heterosis, Insect-resistant donor, Evaluation of insect-resistance
