摘要
目的建立CD4+T淋巴细胞胞内细胞因子染色法,研究艾滋病人在治疗过程中Th1/Th2平衡漂移。方法胞外CD分子染色和胞内细胞因子染色相结合,应用流式细胞术分析CD4+T淋巴细胞的Th1/Th2亚群百分比。结果艾滋病人Th1型细胞百分比的均值在治疗过程中呈阶梯状上升趋势,其与治疗时间在68周内的回归系数为0.741,但无统计学意义(r=0.741,P=0.057);Th2细胞百分比的均值在治疗过程中呈明显的下降趋势,其与治疗时间在68周内的回归系数为0.847,有统计学意义(r=0.847,P=0.016)。结论细胞内细胞因子染色能监测CD4+T淋巴细胞Th1/Th2亚群漂移,对了解艾滋病人治疗效果有重要价值。
Objective To develop an intracellular eytokine staining method and investigate CD4^＋ T cell Th1/Th2 balanee shift in AIDS patients. Methods CD moleetdes staining and intracellular eytokine staining were used synehronously, Flow eytometer was used to analyze the percent of CD4^＋ T cell Th1/Th2 subsets, Results During treatment of AIDS patients, the average percent of Thl cell rised gradually and the average percent of Th2 cell percent deseended obviously, There no significant differenees between the pereent of Thl cell and therapy time of 68 weeks were observed（r = 0.741, P = 0.057）. While signifieant differenee between the percent of Th2 cell and therapy time of 68 weeks was observed （r = 0. 847, P = 0,016）. Conclusion Intracellular eytokine staining method is an excellent teehnologieal platform in monitoring Th1/Th2 balanee shift and the the monitoring of Th1/Th2 balanee shift is valuable for understand the effeet of treatmetn of AIDS patients.
出处
《中国热带医学》
CAS
2007年第10期1737-1739,共3页
China Tropical Medicine
基金
深圳市科技计划项目(200602161)
