摘要
采用RT-PCR扩增的方法,从水稻(Oryza sativa)中克隆获得了1 574 bp的cDNA片段。基因测序和序列同源性分析结果表明,所克隆的基因片段包含1 284 bp的开放阅读框,编码一个含有427个氨基酸的多肽,该蛋白与拟南芥SOS 5蛋白同源性达45.6%,与小麦同源性达71%。
The cDNA fragment of a salt overly sensitive 5 gene in rice （Oryza sativa） was amplificated using RT-PCR technique. The PCR primers were designed according to the blast sequence of SOS5 from Arabidopsis. The fragment was further cloned and sequenced. The result showed that the full-length of cDNA sequence was 1 574 bp, including an ORF of 1 284 bp, which encoded a peptide of 427 amino acids. Compared with the deduced amino acid sequences from Triticum aestivum and Arabidopsis thaliana, the identity were 71% and 45.6%, respectively.
出处
《河南农业科学》
CSCD
北大核心
2007年第9期14-16,20,共4页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关项目(0624440060)
河南省青年骨干教师资助计划(521697)
河南师范大学科研启动费支持课题(052055)
