摘要
基于实验验证的22种大肠杆菌K12的转录因子结合位点序列,分析了转录因子结合位点每一位置的碱基保守性,提出了预测转录因子结合位点的位置权重矩阵打分函数算法(PWMSA)。利用self-consistency和cross-validation两种检验方法对此算法进行检验,self-consistency检验总的预测成功率达到87.59%,cross-validation检验成功率达到85.48%。对基因间序列进行搜索,获得了多个可能的转录因子结合位点。
Bascd on the known transcription factor binding sites in Eacherichia coli K- 12 genome, the conservation analysis of transcription factor binding sites shows that the conservative nuclotide in different sites play a key role in transcription factor binding sites regions, and a novel position - weight scoring matrix scoring algorithm for predicting transcription factor binding sites is presented. By using of the self- consistency test and the 10- fold cross- validation test, the results show that the overall prediction accuracies are 87.59% and 85.48%, respectively. In addition, some possible transcription factor binding sites are predicted by searching for the intergenic regions.
出处
《生物信息学》
2007年第3期117-120,共4页
Chinese Journal of Bioinformatics
基金
国家自然科学基金项目(No.30560039)
