摘要
目的探讨正常幼龄豚鼠眼巩膜组织毒蕈碱受体(M受体)五种亚型的表达。方法2周龄三色豚鼠6只,RT-PCR检测巩膜组织M受体亚型的mRNA的表达,免疫荧光法染色检测M受体的蛋白水平的表达。结果豚鼠巩膜组织中有M受体五种亚型mRNA的表达,其中以M1亚型的表达最高。激光共聚焦显微镜观察发现,在巩膜基质中可见到M受体五种亚型的表达绿色荧光,围绕在成纤维细胞核周围。结论豚鼠巩膜组织中存在M受体五种亚型,提示M受体参与调控眼球的生长。
Objective To investigate the expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs. Methods The scleral tissue was collected from six 2-week-old pigmented guinea pigs to determine mRNA expressions of the muscarinic receptors with RT-PCR. Immunofluorescence was used to observe the protein expressions of the 5 muscarinic receptor subtypes. Results The mRNAs of the 5 muscarinic receptor subtypes were all detected in the scleral tissue, and the mRNA expression was the highest for M1 subtype. Green fluorescence of M1 to M5 subtypes surrounding the fibroblast nuclei were found in the scleral matrix using laser confocal microscopy. Conclusion Five muscarinic receptor subtypes are present in the scleral tissues of immature guinea pigs, indicating the involvement of the muscarinic receptors in eye growth regulation.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第9期1327-1330,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30572005)
美国CMB基金(SUM598677)~~
关键词
受体
免疫荧光
RT-PCR
巩膜
豚鼠
muscarinic receptors
immunofluorescence
reverse transcriptase-polymerase chain reaction
sclera
guinea pigs
