摘要
目的:观察血管成形术后平滑肌细胞增生及再狭窄形成过程,探讨低分子量肝素的抑制作用.方法:以PT-CA导管对家兔髂动脉行血管成形术,于术后不同时间取血管成形段血管,行HE染色及增殖细胞核抗原(PCNA)兔疫组化染色,测量内膜/中膜比值,计数平滑肌细胞的增殖细胞核抗原阳性率.结果:血管成形术造成血管损伤,术后3天即见有少许平滑肌细胞迁移至内膜,至14天时内膜增厚,3、7、14天时中膜平滑肌细胞的增殖细胞核抗原阳性率分别为39.54%、10.78%、3.23%,低分子量肝素组不同时间内膜增厚程度及增殖细胞核抗原阳性率均低于血管成形术组.结论:血管成形术的损伤导致中膜平滑肌细胞增生并向内膜迁移,形成再狭窄病变,低分子量肝素能够部分抑制平滑肌细胞的增生及迁移,减少再狭窄形成.
Purpos: To observe the proliferation of smooth muscal cells (SMCs ) and the formation of restenosis after percutaneous transluminal angioplasty (PTA) and the inhibitive effect of low-molecular weight heparin (LMWH). Methods: The iliac arteries of rabbits were angioplastied by a 2F PTC A catheter, and examined with histomorphological and immunohistological criteria. Results: The wall of artery was injuried in the PTA group: SMCs proliferated in intima at 3 days. and increased at 14 days; the positive percentage of PCNA of medial SMCs were 39. 549.10. 78%, 3. 23% at 3, 7. 14 days respectively. In the LMWH-treated group, the extent of intimal increased and the positive rate of PCNA of SMCs were lower than those in the PTA group. Conclusion :The results indicate that the injury by PTA results in the proliferation and migration of SMCs. and thus form restcnosis . and that LMWH could inhibit the proliferation and migration of SMCs and reduce the formation of restenosis.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1997年第3期273-277,共5页
Chinese Journal of Anatomy
关键词
血管成形术
再狭窄
平滑肌细胞增殖
肝素
动脉
percutaneous transluminal angioplasty (PTA)
restenosis
smooth muscle cclls(SM-Cs)
low molecular weight heparin(LMWH)
