摘要
噻唑蓝(MTT)-四氯化碳萃取比色法是一种快速测定活细胞的方法,通过对反应条件的探索,建立了一种快速的测定细菌活性的方法。结果表明,MTT-四氯化碳萃取法可以有效地测定活菌数,并且与传统的平板稀释法和比浊法的测定结果一致,可以作为代替平板稀释法活菌计数的一种新方法。
Thiazolyl blue tetrazolium bromide(MTT)-carbons tetra-chloride extraction method is a fast method to measure living cell. A kind of fast measurement was built to count the living bacterial cell number by search for the reaction condition. Results showed that MTT-carbons tetrachloride extraction method can measure the living bacterial availably with results in accordance with the traditional dilute plate method and turbidity method. This method can replace dilute plate method and turbidity method to measure living bacterial.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2007年第2期48-50,共3页
Environmental Science & Technology
基金
上海市重点基础研究项目(04JC14072)
上海市生态基金资助
