摘要
利用LCA-agarose固相亲和层析法分离血清中核心岩藻糖化的运铁蛋白（cFuc-Tf），再结合运铁蛋白免疫比浊法，测定了50例正常人、44例良性肝病患者以及19例肝癌患者血清中的核心岩藻糖化运铁蛋白。结果显示正常人、良性肝病患者以及肝癌患者血清中的CFCC-Tf分别为36±34.9mg／L、40．5±34．1mg／L和167．8±104mg／L。经统计学处理，肝癌患者血清中的cFuc-Tf含量较正常人及其它良性肝病患者有极显著的升高。以cFuc-Tf＞140．7mg／L为诊断上限时，诊断肝癌的特异性为98．9％，阳性率为579％，对伴有AFP升高（＞40pg／L）之肝癌，阳性率为100％。血清eFuc-Tf测定可作为肝癌鉴别诊断的一项新指标。
Serum core fucosylated transferrin (cFuc-Tf) was isolated from sera of patients with hepatocellular car-cinoma,benign liver diseases and healthy individuals by immobilized lens culinaris agglutinin (LCA) affinitychromatography column and determined by immunoturbidimetry. It was found that the contents of seracFuc-Tf from fifty healthy individuals,forty four patients with benign liver diseases and nineteen patientswith hepatocellular carcinoma were 36± 34. 9 mg/L,4o. 5± 34. 1 mg/L and 167- 8± 104 mg/L respectively.The results indicate that serum cFuc-Tf of patients with hepatocellular carcinoma is significantly higher thanthat of those with benign liver diseases and healthy individuals. The specificity and the positive rate of cFuc-Tf (over 14O. 7mg/L)were 98. 9 % and 57. 9% for the diagnosis of hepatoma- For the hepatoma patients withhigh serum level of AFP (over 4o μg/L),the positive rate could be 100%. The measurement of serum cFuc-Tf may be useful for the differential diagnosis of hepatoma.
出处
《上海医学检验杂志》
1997年第2期74-76,共3页
Shanghai Journal of Medical Laboratory Sciences
