摘要
目的:验证雪灵芝是否具有抑制大鼠肝癌的功效。方法:实验于2003-09/2004-08在广西疾病预防控制中心SPF级动物实验室完成。选用健康成年SD大鼠160只。按体质量分层随机分为5组:空白对照组、模型组、雪灵芝高剂量组、雪灵芝中剂量组和雪灵芝低剂量组,每组32只。雪灵芝高、中、低剂量组大鼠分别灌胃2.500,1.250,0.625mL/kg雪灵芝溶液,阴性对照组和模型组灌胃等量蒸馏水,1次/d,连续60d。第61天开始雪灵芝高、中、低剂量组和模型组灌胃二乙基亚硝胺溶液,对照组灌胃等量的生理盐水。于停止灌胃90d后各组处死一半受试大鼠(雌雄各半),检测血常规及血清主要生化指标,观察各脏器大体形态改变、脏器的癌变程度。1周后给剩余大鼠灌胃雪灵芝溶液(不含二乙基亚硝胺)。7周后处死余下的一半雄性大鼠,进行相同操作。8周后处死余下的全部大鼠,操作及检测方法同前。结果:纳入的160只SD大鼠,145只进入结果分析,15只脱落。①病理切片检查结果:除阴性对照组,其他各组大鼠肝组织均发生癌变或癌前病变。雪灵芝高、中、低剂量组的癌前病变发生率与模型组相近(P>0.05);癌变的发生率均低于模型组,差异有显著性意义(P<0.05,0.01)。②大体标本检查结果:阴性对照组大鼠肝脏的大体标本均无异常改变,其他各组大鼠的肝脏有些可见表面粗糙等病理改变。模型组大体标本病理改变的阳性率高于雪灵芝高、中、低剂量组,差异有显著性意义(P<0.05)。肉眼观模型组癌变发生率高于其他各组。③其他脏器检查结果:模型组2只大鼠有肝癌肺转移。结论:以较大剂量的二乙基亚硝胺连续灌胃30d可以复制大鼠肝癌模型;雪灵芝对二乙基亚硝胺诱导的大鼠肝癌具有预防和抑制的作用。
AIM： To explore the effect of A.kansuensis on inhibiting rats＇ liver cancer. METHODS： The experiment was carried out in the SPF-grade Animal Laboratory of Guangxi Center for Disease Prevention and Control from September 2003 to August 2004. A total of 160 SD adult rats were randomly divided into five groups according to body weight： blank control group, model group and three dosage groups, each containing 32 rats. The concentrated extraction of A.kansuensis was given by intragastric injection in dosages as different as 2.500, 1.250 and 0.625 mL/kg for 60 days, then the rats were treated with diethylnitrosamine （DENA） for 30 days. While the model group was injected with distilled water and DENA solution respectively. Ninety days later, half of rats were killed （of either gender） to detect the hematological indexes, serum biochemical indexes and the morphology and cancerization of main viscera organs. One week later, the rats were administrated with A.kansuensis （excluding DENA）. Seven weeks later, haft of the remaining rats were executed for the same operations. Eight weeks later, all the remaining rats were killed and managed identically with the above. RESULTS： Among 160 included rats, there were 145 ones involving in the result analysis except 15 Iost.（1）Pathological section examination： Cancerization or precancerous lesion of liver tissues appeared in all the groups except control group; The occurring rate of precancerous lesion in dosage groups was close to that of model group（P 〉 0.05）, but the occurring rate of liver cancer was significantly lower than that of model group （P 〈 0.05 or 0.01）. （2）Gross sample observation： Liver indexes had no abnormal change in control group, while there were many pathological changes found in other groups, such as particles of rough surface. The positive rate of pathological changes appeared in model group was significantly higher than that of dosage groups （P 〈 0.05）. The occurring rates of liver cancer, which was observed
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第38期7553-7555,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
