摘要
分析了安徽产半夏属的滴水珠、鹞落坪半夏、虎掌及半夏３个居群共６个分类群植株的叶和块茎的ＥＳＴ、ＭＤＨ、ＡＤＨ、ＳＯＤ同工酶，结果表明：叶和块茎中ＥＳＴ、ＭＤＨ的谱带在它们之间都有相互区别的特征，可以作为鉴定原植物及其产地的生化指标。四种同工酶的ＵＰＧＭＡ和Ｆｕｚｙ聚类分析显示鹞落坪半夏和半夏的遗传一致度高，且遗传距离小，它们的遗传差异只能在种内居群间与形态特征的比较相结合，作者认为鹞落坪半夏只是半夏在水肥条件较好，人类干扰较小环境下生长的一特殊居群，而不是一新种，虎掌和其它分类群的遗传距离最大。
The isozyme of esterase,malate dehydrogenase,alcohol dehydrogenase and superoxide dismutase of Pinellia,including Pinellia cordata,P.ternata,P.yaoluopingensis,P.pedatisecta and 2 special populations of P.ternata from Anhui province were analyzed.The results indicated the patterns of esterase and malate dehydrogenase isozyme had different features between the taxa,and could be used as biochemical indexes for identifying these plants and their origins.Cluster analysis of 4 isozyme,applying the methods of UPGMA and Fuzzy,showed that P.ternata and P.yaoluopingensis had the highest genetic identity and lowest genetic distance,this genetic distance could be in the level of populations of P.ternata. Except for the morphological variation between populations,Pinellia yaoluopingensis was considered as a special population of P.ternata,which growing in the place of less human disturbs and has higher level of water and nutrient supply,and not as an independent species.P.pedatisecta has a higher genetic distance compared with other taxa.
出处
《安徽农业大学学报》
CAS
CSCD
1997年第3期291-295,共5页
Journal of Anhui Agricultural University
关键词
半夏属
同工酶
电泳分析
inellia Isozyme Electrophoretic analysis
