摘要
目的:观察热休克蛋白70(HSP70)在人骨肉瘤组织中的表达。方法:采用免疫组化SP法检测55例骨肉瘤组织,其中骨母细胞型24例,软骨母细胞型20例,纤维母细胞型11例,以及30例正常骨组织中HSP70的表达情况。结果:HSP70在正常骨组织与骨肉瘤组织中的表达率分别为20%(6/30)及96.4%(53/55),平均阳性细胞率分别为0.53%和95.57%,两者差异具有统计学意义(P<0.01),在骨母细胞型、软骨母细胞型与纤维母细胞型中阳性表达率分别为98.65%,92.37%,90.36%,三者之间差异无统计学意义。HSP70在骨肉瘤中的表达与病理分级有明显相关性(P<0.05),与临床分期无关(P>0.05)。结论:HSP70在人骨肉瘤组织中高表达,可作为判断恶性程度的重要指标以及肿瘤免疫的重要靶位。
Objective： To detect the expression of heat shock protein 70（HSP70） in osteosarcoma and explore its role in anti-tumor immunity. Methods： Fifty-five cases of osteosarcoma and thirty cases of normal bone tissue were detected for expression of HSP70 by means of immunohischemistry （IHC）. Results： The expression rate of HSP70 was 96.4%（53/55） in osteosarcoma, but 20%（6/ 30）in normal bone tissue. The difference of average positive cell rate in osteosarcoma and normal bone tissue was significant（P〈0.01）. The positive cell rate in the three types was 98. 65%, 92.37% and 90.36% respectively, and the difference had no statistical significance （P〉0. 05）. The expression degree of HSP70 in osteosarcoma maybe relate to histolobical grade （P〈0.05）, but not to the clinic stage （P〉0.05）. Conclusion： HSP70 is over expressed in osteosarcoma and it maybe plays a contributive role on the pathogenesis and development in human osteosarcoma.
出处
《武汉大学学报（医学版）》
CAS
2007年第5期617-620,共4页
Medical Journal of Wuhan University
基金
湖北省科技攻关项目(编号:2006AA412C50)
