摘要
目的探讨大鼠肾缺血再灌注时肺损伤的病理变化,并观察促肝细胞生长素(Hepatocyte Growth-promoting Factor,pHGF)对肺损伤的影响。方法雄性Sprague-Dawley大鼠32只,随机分为假手术对照组(Ⅰ组)、缺血再灌注组(Ⅱ组)、缺血再灌注前pHGF干预组(Ⅲ组)和缺血再灌注后pHGF干预组(Ⅳ组)。用无损伤动脉夹钳夹大鼠双侧肾蒂45分钟制成肾缺血再灌注损伤(renal ischemia reperfusion in-jury,RIRI)模型。假手术组只暴露双肾,不钳夹肾蒂。Ⅲ组和Ⅳ组分别在术前和术后腹腔注射pHGF50mg/kg。检测术后12h血清IL-1β和Scr的浓度及肺组织干湿重比变化,病理切片观察肺组织病理学改变。结果Ⅰ组血清IL-1β、Scr水平均为最低,Ⅱ组各值均显著升高,两组比较各项均有显著差异(均P<0.01),Ⅲ组和Ⅳ组各项数值较Ⅱ组均有一定程度的降低(均P<0.01)。Ⅱ组肺组织干湿重比值较Ⅰ组明显降低,Ⅲ组和Ⅳ组均较Ⅱ组上升,差异有显著的统计学意义(均P<0.01);缺血再灌注12h肺组织病理改变最严重,有明显肺泡内和肺间质水肿及炎症细胞的浸润。pHGF干预后,肺脏形态学改变明显减轻;各项指标在Ⅲ组和Ⅳ组之间比较无显著差异(P>0.05);结论肾缺血再灌注后释放的细胞因子IL-1β可致急性肺损伤,pHGF对其既有保护作用又有治疗作用。
Objective To investigate Interleukin- 1β（IL- 1β） expression and pathological lesions of lungs in rats with renal ischemia reperfusion and the effects of Hepatocyte Growth- promoting Factor（pHGF） on them. Methods Thirty - two male Sprague- Dawley rats were divided into four groups （n = 8） ： Sham - operated control group（Group Ⅰ）, renal IR control group（Group Ⅱ ）,one experimental group injecting pHGF（50mg/ kg, intraabdominal injection） before renal IR injury（Group Ⅱ） ;and another experimental group injecting pHGF （50mg/kg, intraabdominal injection） after renal IR injury（Group Ⅳ）. The animals with renal IR injury exposed to 45 - rain bilateral renal pedicle clamping. All the rats in Group Ⅰ and Ⅱ were intraabdomially injected equal volume of physiological saline（0.8ml） at the time when the rats in experimental groups were administered 0.8ml pHGF. Twelve hours after IR injury,samples for serum and the left kidney and lung of each animal were taken. The serum sample was for analysis of expression of creatinine（Scr） and interleukin- 1β（ IL- 1β）, and the kidney and lung in each group for histologic examination. While lung dry to wet weigh ratio were respectively examined, Results The level of blood Ⅱ- 1βand Scr in group Ⅱ were significantly higher than those in group Ⅰ（P〈0. 01）, The level of blood Ⅱ - 1βand Scr in groups Ⅲ and Ⅳ were decreased significantly by comparison with those in group Ⅱ（P 〈 0.01）. Lung dry to wet weight ratio in group Ⅱwas markedly lower than that in group Ⅰ,and those in groups Ⅲ and were significantly increased compared with group Ⅱ （P 〈 0.01）. The pathological lesions of lungs were most severe at IR 12h, After rats were given pHGF, lungs injury were significantly attenuated （ P 〈 0.01）. No statistical significance was detected between Group Ⅲ and Group Ⅱ concerning any detected parameters in the study（P〉0.05）, Conclusion this study demonstrate that renal IR cam result in l
出处
《济宁医学院学报》
2007年第3期185-188,共4页
Journal of Jining Medical University
关键词
促肝细胞生长素
肾
缺血再灌注
肺
白细胞介素-1Β
Hepatocyte Growth - promoting Factor
Kidney
Ischemia reperfusion
Lung
Interleukin - 1β
