摘要
犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬腺病毒(CAV)是引起犬传染病的主要病原体,严重危害犬的健康,基因疫苗具有安全和克服幼犬母源抗体对主动免疫干扰的优点.根据CDV的H基因、CPV的VP1基因、犬2型腺病毒(CAV-2)F基因的核苷酸序列,用PCR或RT-PCR方法扩增克隆目的基因,分别构建了pIRCDV-H,pIRESVP1和pcCAV-F 3个真核表达质粒并进行了序列验证,用3种免疫质粒经纯化后混合对15只犬进行免疫,所有接种混合基因疫苗的犬,2次免疫后即可产生较高的抗体水平,攻毒后大部分犬能抵抗3种强毒的攻击,对照犬发病严重,证明所构建的核酸疫苗在一定程度上可抵抗强毒的攻击.
The canine distemper virus, canine parvovirus, canine adenovirus are three kinds of diseases of canine. The CDV H gene, CPV VP1 gene, CAV F gene were clonded by PCR or RT-PCR. Recombinant plasmid pIRCDV-H, pIRESVP1, pcCAV-F were constructed and sequenced to confirm its rightness. Fifteen dogs were injected at 9 weeks of age with three kinds plasmid of pIRCDV-H,pIRESVP1 ,pcCAV-F.All dogs were challenged with virulent CDV, CPV, CAV after the third injection. NAV vaccinated dogs showed a high IgG titer after the second injection and were protected against infection, while the control dogs were fully susceptible. That vaccines can protect some dogs against virulent was demonstrated.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第5期529-535,共7页
Journal of Yunnan University(Natural Sciences Edition)
基金
军队"十一五"医药卫生青年学者项目资助(06Q035)
关键词
犬瘟热H基因
犬细小病毒VP1基因
犬腺病毒F基因
基因免疫
：canine distemper virus H gene
canine parvovirus VP1 gene
canine adenovirus-2 fiber gene
gene immunization
