摘要
目的观察表皮生长因子对原始生殖细胞来源的小鼠胚胎干细胞分离克隆的影响。方法以小鼠胎儿成纤维细胞为饲养层,从原始生殖细胞中分离克隆小鼠胚胎干细胞的过程中,在基础细胞培养液中加入不同浓度的表皮生长因子,观察小鼠胚胎干细胞集落形成数和各组的最高传代数。结果表皮生长因子添加与否对原始生殖细胞来源的小鼠胚胎干细胞原代培养无显著的影响,而对后继培养有显著影响。结论在原始生殖细胞来源的小鼠胚胎干细胞分离的过程中,可以不添加表皮生长因子,而在胚胎干细胞的后续传代中,尤其对于较高代细胞的克隆过程中添加表皮生长因子有利于细胞的生长。
Objective To investigate the effects of the EGF on isolation and cloning of mouse embryonic stem - like cells from PGCs. Methods EGF with different density were added to the culture medium of cells . After a culturing period ,the cloning number of mouse embryonic stem -llke cells and the topmost passage would be observed and registered. Results There was not obvious effects on the primary culture of mouse ESC and was distinct on the later passage if EGF were used. Conclusions In the primary passage of mouse ESC may survive without EGF,and the impact of the existence of EGF was better than the one of the inexistence to the cloning of mouse ESC.
出处
《医学研究杂志》
2007年第9期23-25,共3页
Journal of Medical Research
基金
国家自然科学基金(39970363)
国家973(G1999054300)资助项目
泰山医学院科研基金资助项目(2006年度)
关键词
表皮生长因子
小鼠胚胎干细胞
分离克隆
Cytokines
Mouse embryonic stem -like cells
Isolation and cloning
