摘要
根据鸭生长激素基因编码区及调控区的序列设计8对引物,利用PCR-SSCP方法对北京鸭、西湖野鸭、樱桃谷鸭、金定鸭、山麻鸭、荆江鸭、绍兴鸭、缙云麻鸭等8个鸭种进行单核苷酸多态性分析。结果共发现3个突变位点,分别为230处(C→G)、244处(C→A)和3 701处(C→T)。前两处突变位于5′调控区,3 701处突变位于编码区第4外显子,但该编码区的突变是沉默突变,3′调控区表现了高度的保守性。统计结果发现:⑴在5′调控区基因座上,金定鸭的等位基因B频率显著高于其他品种;⑵在外显子4基因座上,基因型频率的分布与品种有关,且肉用型鸭的CC基因型频率显著高于蛋用型。可以推测,本研究所检测到的基因座可能与生产性能相关。
The single nucleotide polymorphism （SNP） of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Cherry Valley Meat ducks, Jinding ducks, Shan Partridge ducks, Jingjiang ducks, Shaoxing ducks and Jinyun Partridge ducks. Eight pairs of primers for coding and regulation regions of GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method. Three SNPs were found among individuals, which were 230（C→G）, 244（C→A） in 5＇-UTR and 3 701（C→T） in exon 4, respectively. However, the mutation at 3 701 was a silence mutation. And the 3＇-UTR represented its own high conservativeness. The analysis results showed that. （1） At locus of 5＇-UTR, the frequency of B allele in Jinding ducks was significantly higher than that in other breeds. （2）At locus of exon 4, the frequencies of genotypes in different breeds were significantly different, and the frequency of CC genotype in meat-type ducks were significantly higher than that in laying-type ducks. According to the results above, we can conclude that these SNPs may have relationship with some productive traits of ducks.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第9期907-912,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
鸭
生长激素基因
单核苷酸多态性
duck
growth hormone gene
single nucleotide polymorphism
