摘要
目的观察CpG-ODN体外对慢性乙型肝炎患者PBMC的免疫刺激效应。方法CpG-ODN体外刺激慢性乙肝患者外周血单个核细胞(PBMC),用ELISA检测培养液中IFN-α的水平;将不同比例的PBMC培养上清与HepG2.2.15细胞共孵育4和8d后,用ELISA和荧光定量PCR法,分别检测培养上清液中HBsAg、HBeAg和HBV DNA的水平;MTT法观察活化的PBMC培养上清液对HepG2.2.15的杀伤作用。结果CpG-ODN可有效诱导慢性乙肝患者PBMC分泌IFN-α,增强其活化的PBMC培养上清液对HepG2.2.15的杀伤作用;CpG-ODN本身虽不能直接抑制HBV的复制,但可通过其活化的PBMC的培养上清抑制HepG2.2.15细胞产生HBsAg、HBeAg和HBV DNA。结论CpG-ODN可有效激活慢性乙肝患者的免疫细胞,发挥抗病毒效应。
Objective To investigate the effects of synthetic oligodeoxynucleotides （ODN） containing unmethylated CpG dinucleotides （CpG-ODN） on enhancing immune response in PBMCs of patients infected with hepatitis B virus （HBV）. Methods CpG-ODN was co-cultured with peripheral blood mononuclear cells （PBMCs） obtained from healthy controls and individuals infected with HBV, and the levels of IFN-α in the culture supernatants were measured by ELISA. The culture supernatants of PBMCs were added to HBV-transfected HepG2.2.15 cells with various ratios. Levels of HBsAg, HBeAg, and HBV DNA in culture supernatant of HepG2.2.15 cells were detected by ELISA and fluorescence quantitative PCR at day 4 and 8. MTT method was used to detect lytic activity of HepG2.2.15 cells mediated by the culture supernatants of CpG-ODN-stimulated PBMCs. Results CpG-ODN induced high amounts of IFN-α production. The lysis of HepG2.2.15 cells mediated by the culture supernatants of CpG-ODN-stimulated PBMC was increased. CpG-ODN alone could not inhibit HBV replication directly, but the culture supernatant of PMBCs activated with CpG-ODN could remarkably inhibit the production of HBsAg, HBeAg and HBV DNA by HepG2.2.15 cells. Conclusion CpG-ODN can efficiently increase the immune response of chronic hepatitis B patients and enhance the antiHBV effects.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第5期506-509,共4页
Immunological Journal
基金
湖南省科技攻关计划项目资助课题(04SK3040-3)
长沙市科技项目(K05ST002-12)
