摘要
目的建立高效液相色谱(HPLC)法测定双醋瑞因有关物质和含量。方法采用十八烷基硅烷键合硅胶为填充剂,乙腈-水(47:53,用醋酸调节pH值至2.7)为流动相,检测波长为254 nm。结果双醋瑞因与有关物质分离良好,线性范围为12～100μg.mL-1(r=1.000 0,n=7),最低检出量为0.5 ng。结论该法简单快速,专属性强,结果准确,可用作双醋瑞因含量测定和有关物质检查的方法。
Objective To establish HPLC method for the determination on the content of diacerein and its related substances. Methods A C18 column was used , the mobile phase of acetonitrile-water （adjust pH to 2.7 with acetic acid） （47 ： 53） and the detection wavelength was 254 nm. Results Diacerein and related substances could be well isolated. The calibration curve was linear in the range of 12 - 100 μg·min^-1 with r = 1. 000 0 （ n = 7 ）. The detection limit was 0.5 ng. Conclusion This method is simple, rapid, accurate and suitable for the determination on the content of diaeerein and its related substances.
出处
《医药导报》
CAS
2007年第9期1077-1078,共2页
Herald of Medicine
