摘要
目的:观察复方丹参滴丸对高糖/高胰岛素诱导兔胸主动脉血管平滑肌细胞(VSMCs)增殖的抑制作用及其机制。方法:组织贴块法原代培养VSMCs,用高糖/高胰岛素诱导细胞增殖。设正常对照组,模型组,一氧化氮合酶(NOS)抑制剂N-硝基-L-精氨酸甲酯(L-NAME)组,吡格列酮以及联合L-NAME组,复方丹参滴丸浸膏低、中、高剂量干预组,低、中、高剂量复方丹参滴丸浸膏合用L-NAME组。采用四甲基偶氮唑盐(MTT)比色法测定VSMCs增殖,试剂盒检测细胞总NOS及一氧化氮(NO)含量。结果:高糖/高胰岛素可诱导VSMCs增殖,表现为模型组VSMCs含MTT溶解物的吸光度(A)值显著高于正常对照组,总NOS活性及NO含量显著低于正常对照组(P均<0.01);与模型组比较,吡格列酮及中、高剂量复方丹参滴丸浸膏组VSMCs含MTT溶解物的A值均明显降低,总NOS活性及NO含量明显增加,并且能被L-NAME部分阻断(P<0.05或P<0.01);低剂量复方丹参滴丸浸膏组上述结果比较差异均无显著性(P均>0.05)。结论:复方丹参滴丸浸膏可以通过NO介导而抑制VSMCs增殖。
Objective： To study the anti - proliferative effects and possible mechanisms of compound Danshen dripping pill （复方丹参滴丸） on rabbit aortic smooth muscle cells during the insulin resistance state. Methods： The primary vascular smooth muscle cells （VSMCs） were cultured by planting rabbit thoracic smooth muscle pieces. The proliferation of VSMCs was induced by high concentrations of glucose and insulin. The rabbits were divided into following groups： a model group, a normal control group, treated groups including a pioglitazone （PIO） group, compound Danshen dripping pill （low, middle and high dose） subgroups and the various treated groups with respectively the addition of nitric oxide synthase （NOS） inhibitor N-nitro-L-arginine methylester （L - NAME） groups and L - NAME group. Cell proliferation was measured by methyl thiazolyl tetrazolium （MTT） assay, total NOS activities and nitrogen oxide （NO） content of VSMCs were determined by assay kits,respectively. Results： The proliferation of VSMCs was markedly induced by high concentrations of glucose and insulin, representing that the observation degree （A value） of the MTT soluble substance in VSMCs in the model group was higher than that of normal control group, but the total content and activity of NOS were lower than those of normal control group （all P〈0.01）. Compared with the model group, the observation degree （A value） of MTT soluble substance in VSMCs was markedly decreased, and the total NOS activities and NO content of VSMCs were significantly increased in the PIO group, middle and high compound Danshen dripping pill groups, and the above effects could be partially blocked by NOS inhibitor L- NAME （P〈0. 05 or P〈0.01）; while there were no significant differences in above indexes in low compound Danshen dripping pill group （all P〉0.05）. Conclusion： In the valid concentration range, compound Danshen dripping pill inhibits proliferation by involving in NO pathway in cultured VSMCs duri
出处
《中国中西医结合急救杂志》
CAS
2007年第5期313-316,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
复方丹参滴丸
血管平滑肌细胞
增殖
一氧化氮
一氧化氮合酶
compound Danshen dripping pill
vascular smooth muscle cells
proliferation
nitrogen oxide
nitric oxide synthase
