摘要
以玻璃纸做材料,用毛细吸管分离单卵囊,通过显微镜"重复"鉴定法,对柔嫩艾美耳球虫(Eimeria tenella)进行分离。单卵囊感染20只鸡,在感染后5 d～15 d,用饱和盐水漂浮集卵法进行检测。结果显示,有15只鸡粪便中检出卵囊,经鉴定均为所要分离的虫种(E.tenella),感染成功率为75%,准确率达100%。另外,应用改进后的玻璃纸单卵囊分离技术还成功分离到毒害、巨型、堆型、布氏、和缓和早熟艾美耳球虫的单一虫种。结果表明,改进后的单卵囊分离技术简单易行,感染方便,成功率和准确率都较高,而且所用材料易于获得,消毒方便。
Eimeria species were isolated successfully from chickens in Lanzhou,Gansu province,by single oocyst separating technique with plastic paper.Under experimental conditions,20 chickens were infected with single E.tenella oocyst.After 5～15 days,the oocysts in the feces were detected through saturated brine flotation.amoug which the oocysts of E.tenella were detected possitive in 15 chickens.The results show that the method used in this study is simple,convenient and effective with 75 percent of success rate and 100 percent of accuracy.Moreover,the material（plastic paper） is easily available and sterilizing.indicating that the technique with plastic paper charicterized with being practical,simplicity,convenience,higher success rate and accuracy.
出处
《动物医学进展》
CSCD
2007年第8期27-29,共3页
Progress In Veterinary Medicine
基金
甘肃省生物技术研究与开发专项(GNSW03-2)
关键词
鸡
球虫
单卵囊分离技术
感染
chicken
Eimeria
single oocyst isolation
infection
