摘要
目的应用6-OHDA制作帕金森病(PD)大鼠模型,并对不同剂量6-OHDA制作的模型进行综合评价。方法取雄性SD大鼠70只,随机分成12μl单点、8μl单点、4μl单点、8μl双点和对照组,采用立体定向法,分别行左侧纹状体区相应剂量6-OHDA单点及双点注射,检测大鼠行为学变化及黑质多巴胺(DA)能神经元变化。结果双点组大鼠模型病死率高于其余各组,12μl单点组大鼠模型成功率高于其余各组,行为学检测见各单点组随6-OHDA剂量增加,其异常行为出现率增高;免疫组化检测各单点组间存在显著差异,但12μl单点组与双点组之间无显著差异。结论纹状体区对6-OHDA损伤存在剂量依赖性,12μl单点注射方法优于双点注射法。
Objective The aim of this study is to establish a stable and easy to perform Parkinson＇s disease rat model by using 6-OHDA.Methods 70 male SD rats were used in this study. Different doses（4μl,8μl,12μl） of 6-OHDA were injected to the left striaturn area by employing single-injection strategy under the facilitation of stereotaxis equiprnents.8gl of 6-OHDA was injected by employing double-position injection strategy to act as a postitive control grop. The control group was injected with the same volume of saline. The ethological alterations and the suhsbstantia nigra dopaminergic neuronal changes were investigated to compare the profiles of PD models induced by different doses and by single or double-position injection methods of 6-OHDA at the dosage of 8μl.Results The motality rate of rats employing double-position strategy was higher that other groups. The 12μl group rats showed increased successful model rates in comparision with other groups＇ rats. No significant ethological changes were in either groups. Conclusion In this study a stable and easy to perform PD rat model was established successfully by employing single-position injection strategy of 6-OHDA at adose of 12μl to stria- turn area.This study builds a strong experimental and theoretical basis on making PD animal models with better successful rate and less mortality rate and will facilitate the further research on PD.
出处
《四川医学》
CAS
2007年第8期818-820,共3页
Sichuan Medical Journal
基金
国家自然科学基金资助项目(No.30371461)
