摘要
以香蜂花茎段为外植体,MS为基本培养基,设计不同浓度的6-BA、NAAI、BA等单因子和不同比例的细胞分裂素和细胞生长素组合试验。结果表明:适宜不定芽诱导分化的培养基为MS+6-BA 2.5 mg/L+NAA 0.2 mg/L;适宜诱导生根的培养基为1/2MS+IBA 2.0 mg/L。
Stalk segments of Melissa officnalis were explants on MS medium supplemented with plant hormone in different concentrations.The results showed that MS medium supplemented with 6-BA 2.5 mg/L and NAA 0.2 mg/L was the most effective medium for the differentiation and multiplication of the shoots.The optimal rooting medium was 1/2MS+IBA 1.0 mg/L.
出处
《湖南农业科学》
2007年第4期26-28,共3页
Hunan Agricultural Sciences
基金
河南省创新人才工程项目
河南科技学院博士基金资助项目(5046)
关键词
香蜂花
组织培养
激素
Melissa officnalis
tissue culture
plant hormone