摘要
目的探讨PDX-1蛋白对棕榈酸诱导的大鼠胰岛凋亡的保护作用。方法PDX-1基因克隆及表达质粒的构建,PDX-1蛋白的表达、纯化和鉴定,PDX-1蛋白保护棕榈酸引起的胰岛细胞凋亡的研究。结果PDX-1蛋白可以延缓胰岛细胞的自然凋亡过程,保护其三维形态;可以降低处理后的胰岛Caspase-3活性,同时可以增加葡萄糖刺激胰岛素释放,PDX-1蛋白干预后的棕榈酸组(1.560±0.074)uIU/mL,与对照组(1.426±0.056)uIU/mL相比有显著差异(P<0.05)。结论表明PDX-1蛋白可以减少棕榈酸诱导的胰岛凋亡,保护β细胞功能。
Objective To study whether the PDX-1 protein has antiapoptosis effects induced by palmitic acid on rat islet of Langerhans. Meth- ods PDX-1 gene coloning and expression plasmid construction.PDX-1 protein expression, purification and identification.Study of PDX-1 protein counteracts apoptosis on primary rat islet of Langerhans induced by palmitic acid. Results PDX-1 protein could delay the natural apoptosis of rat islet; decrease caspase-3 activity; PDX-1 protein could promote an increase in insulin secretion （1.560 ± 0.074）ulU/mL with compared group （1.426 ± 0.056）ulU/mL （P 〈 0.05）. Conclusion PDX-1 protein can counteract apoptosis on primary rat islet of Langerhans induced by palmitic acid.
出处
《中国现代医生》
2007年第09S期21-22,F0003,共3页
China Modern Doctor
关键词
PDX-1蛋白
蛋白的表达和纯化
凋亡
棕榈酸
大鼠胰岛
PDX-1 protein
Protein expression and purification
Apoptosis
Palmitic acid
Islet of Langerhans
