摘要
目的:研究维甲酸对缺氧缺血性脑损伤(HBD)新生鼠内源性神经干细胞(NSCs)的激活作用,为HIE早期干预治疗探索新的思路。方法:将75只7日龄新生SD乳鼠随机分为正常组、HBD生理盐水组及HBD维甲酸治疗组,根据处死时相点每组分成1、3、7、14、21天5个小组,每小组5只。结扎7日龄新生SD乳鼠左颈总动脉后置于8%氧浓度的低氧环境中2.5h建立HBD模型。HBD维甲酸治疗组SD鼠腹腔注射维甲酸,HBD生理盐水组腹腔注射生理盐水。采用HE、免疫组织化学染色以及光镜技术分别对3组SD大鼠脑组织中的NSCs进行检测,Nestin阳性细胞标志神经干细胞。结果:3组SD大鼠脑组织均存在NSCs,生理盐水组3天NSCs数达高峰,较正常组提高了Nestin阳性细胞的数量(P<0.05);,维甲酸治疗组7天NSCs数达高峰,与生理盐水组相比,在7、14、21天时间点能显著提高Nestin阳性细胞的数量(P<0.05);3组SD大鼠脑组织Nestin阳性细胞数均随着年龄增大而逐渐下降。结论:维甲酸可显著促进HBD内源性NSCs的增殖,为HIE早期干预治疗提供了新的思路。
Objective： To investigate the activation effect of retinoic acid （ RA ） on endogenous neural stem cell after hypoxicischemic brain injury （HBD） in newborn rats and to the explore new means for early intervention pretreat hypoxicischemic encephalopathy （HIE） . Methods： 75 neonatal rats aged 7 d were divided randomly into normal group, hypoxic- ischemic NS control group and RA-treated group. According to the time of sacrefice [first day （1 d） , third day （3 d） , the seventh day （7 d） , the fourteenth day （14 d） and the twenty - first day （21 d） ], 25 rats of every group were further divided randomly into five groups, with 5 rats in each subgroup. The HBD mode establish that left common carotid artery of the neonatal rats in hypoxic - ischemic group was ligated and subjected to inhalation of 8% oxygen for 2. 5 h. intraperitoneal injection RA in treated group and NS in NS control group NSCs from brain tissues of the rats of the three groups were determined with HE staining and immunohistochemicalmethod under light microscope. Nestin cell mark NSCs. Results： NSCs existed in brain tissues of rats in all the three group, control group peaked at 3 days, the numbers of NSCs were much more than those in rats of normal group at third day （P 〈0. 05） . RA - treated group peaked at 7 day, the numbers of NSCs were much more than those in rats of control group. At the seventh day and the fourteenth day and the twenty - first day （P 〈 0. 05）, . the numbers of NSCs fall off from rats age accrete. Conclusion ： Our results are indicate that RA can promote the proliferation of endogenous NSCs after HBD and provide early intervention pretreat new means for HIE.
出处
《中国妇幼保健》
CAS
北大核心
2007年第26期3750-3753,共4页
Maternal and Child Health Care of China
关键词
维甲酸
缺氧缺血性脑病
神经干细胞
Retinoic acid
Hypoxicischemic encephalopathy （HIE）
Neural stem cell
