摘要
目的:建立复发性口疮(RAU)大鼠动物模型,探讨T淋巴细胞亚群与RAU发病机制的关系及观察纳米金银花对复发性口疮大鼠模型的免疫调节作用。方法:用大鼠口腔粘膜抗原乳化液注射方法建立RAU大鼠动物模型并观察实验大鼠的口腔表现。将溃疡大鼠随机分为4组:阴性对照组、纳米金银花组、左旋咪唑组、金银花水煎剂组。分别于成模后、给药后大鼠眼眶采血,免疫组化染色,CD4+、CD8+细胞计数并计算其比值。结果:1实验大鼠于最后一次抗原注射6d后开始出现口腔溃疡。RAU大鼠CD4+、CD4+/CD8+细胞量较正常大鼠降低。2实验大鼠给药后,各治疗组外周血CD4+、CD4+/CD8+均有回升,较阴性对照组有显著性差异(P<0.05),纳米组与左旋咪唑组相比无统计学意义,与金银花组相比有显著性差异(P<0.05)。结论:RAU的发病与T淋巴细胞亚群的失衡有关。纳米金银花对复发性口疮大鼠有一定的免疫调节作用,其作用优于相同剂量的金银花水煎剂,而与左旋咪唑无差异。
Objective： To explore the association %etween T-lymphocyte subsets and pathogenesis of RAU by using the rat recurrent oral ulceration（RAU） model and to detect the effect of Nano-Lonicera on peripheraic blood T-lymphocyte subsets in RAU rats. Methods ：We established RAU model by injecting galacta of ral oral mucosa and observed oral situation of rats. We divided the ulcerative rats into four groups ：negative group ,Nano-Lonicera group ,Levamisole group and Lonicera group. CD4＋ and CD8＋ T- lymphocyte were dyed by CD4＋ and CD8＋ Immunohistochemistry of Kits and counted the cells. Results：① The experimental rats had uleration afer 6 days after the last injection. The CD4＋ and CD8＋ T- lymphocytes in RAU rats were depessed. @Compared with negative group,the CD4＋ T-lymphocyte and CD4＋/CD8＋ were improved （P〈 0. 05） . There was no difference between Levamisole group and Nan-oLonicera group,but a notable diference between Nano-Lonicera and Lonicera （P〈0. 05） . Conclusion. The imbalance of T-lymphocyte subsets in RAU contributes to RAU incidence. Nano-Lonicera had the immune regulation in RAU rats. The efficacy of Nano-Lonicera is better than Lonicera and the same as Levamisole.
出处
《黑龙江医药科学》
2007年第4期29-30,共2页
Heilongjiang Medicine and Pharmacy
基金
生物医学材料省级重点实验室资助项目
