摘要
从皮革厂、畜肉市场、屠宰场等处采样,分离得到49株明胶水解活性的细菌。经活性平板初筛、牛皮消化试验和活性测定,筛选得到一株胶原蛋白酶活性为35.97u/mL的菌株。经形态学观察、生理生化特性和16SrDNA序列分析,鉴定该菌株为短小芽孢杆菌。
Forty-nine bacterial strains with glutin hydrolyzation activity were isolated from the samples of tannery, meat mar-ket and slaughterhouse.By screening media,fresh calfskin decomposition and activity detection,one strain was screened with col-lagenase activity about 35.97u/mL.The morphology,physiology biochemistry characteristic analysis and 16S rDNA sequencing,it is identified as a bacillus pumilus.
出处
《中国皮革》
CAS
北大核心
2007年第17期16-19,共4页
China Leather
基金
四川农业大学青年科技创新基金项目资助
关键词
胶原蛋白酶
短小芽孢杆菌
分离
鉴定
collagenase
bacillus pumilus
isolation
identification
