摘要
目的探讨缬沙坦对糖尿病大鼠脑组织氧化应激的影响。方法Wistar大鼠24只,随机分为正常对照组(NC组)、糖尿病组(DM1组)、糖尿病缬沙坦治疗组(DM2组),每组8只。用链脲佐菌素诱发糖尿病大鼠模型制模成功后,DM2组予以缬沙坦40mg/(kg.d)灌胃,共12周。12周后测定各组质量、血糖,Morris水迷宫试验观察大鼠认知功能,比色法测定脑组织中羟自由基(OH-)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,荧光实时定量反转录-聚合酶链反应检测尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶p47phoxmRNA表达。结果(1)第12周未DM1组和DM2组质量明显轻于NC组(均P<0.01),血糖明显高于NC组(均P<0.01);DM1组与DM2组间差异无统计学意义。(2)DM1组、DM2组逃避潜伏期较NC组明显延长(P<0.01,P<0.05),DM2组较DM1组明显缩短(P<0.01)。(3)DM1组OH-、MDA水平显著高于DM2组和NC组(均P<0.01),而SOD活性显著低于DM2组和NC组(均P<0.01)。(4)NADPH氧化酶p47phox mRNA的表达量DM1组是NC组的4.89倍,DM2组是NC组的2.67倍,DM2组是DM1组的54.5%;各组间差异有统计学意义(均P<0.01)。结论缬沙坦能提高SOD的活性,降低OH-、MDA的活性和NADPH的表达,抑制机体的氧化应激水平,改善认知功能。
Objective To study the effects of Valsartan on oxidative stress in brain tissue of diabetic rats. Methods Diabetic Wistar rats were induced by streptozotocin （STZ） and were randomly divided into diabetic group and Valsartan treatment group. In Valsartan treatment group, the rafts received Valsartan 40 rag/（ kg · d） for 12 weeks by intragastric administration. Then blood glucose and body weight were measured. The cognition ability of rats was assayed with Morris water maze test. The activities of superoxide dismutase （SOD） , maleic dialdehyde （MDA） and hydroxy radical （ OH ^- ） were detected by chromatometry. Quantitative real-time RT-PCR was performed to detect the expression of NADPH oxidase p47phox mRNA. Results （ 1 ） Compared with normal control group, the body weight in diabetic group and Valsartan treatment group decreased significantly （both P 〈0. 01 ） , however the level of blood glucose increased significantly （both P 〈 0.01 ）. There was no significant difference between diabetic group and Valsartan treatment groups. （2） Morris water maze test showed that the escape latency was longer in diabetic group and Valsartan treatment group than in normal control group （ P 〈 0. 01, P 〈 0. 05 ）, and it was shorter in Valsartan treatment group as compared with diabetic group （ P 〈 0.01 ）. （ 3 ） The levels of OH- and MDA in diabetic group were much higher than those in normal control and Valsartan treatment groups （both P 〈 0. 01 ） , while the activity of SOD decreased （both P〈0. 01 ）. （4） The level of NADPH oxidase p47phox mRNA in diabetic group was 4.89 times higher than normal control group, and the level in Valsartan treatment group was 2.67 times higher than normal control group. The level in Valsartan treatment group was 54. 5 percent of diabetic group （ all P 〈 0. 01 ）. Conclusions Valsartan can increase the activity of SOD and decrease the levels of OH^- and MDA and the express of NADPH. So it may inhibit oxidative stress level o
出处
《临床神经病学杂志》
CAS
北大核心
2007年第4期292-294,共3页
Journal of Clinical Neurology
关键词
缬沙坦
糖尿病
氧化应激
Valsartan
diabetes
oxidative stress
