摘要
目的研究牙周病致病菌牙龈卟啉单胞菌脂多糖(LPS)对体外培养的人牙周膜成纤维细胞(hPDLF)胶原吞噬作用的影响。方法将不同质量浓度的LPS加入体外培养的hPDLF48h后,采用荧光定位术和流式细胞技术检测hPDLF胶原吞噬率的变化。结果LPS导致hPDLF胶原吞噬率显著增加(P<0.05)。结论牙龈卟啉单胞菌脂多糖具有促进hPDLF吞噬胶原的作用,可能是牙周组织破坏机制之一。
Objective To investigate the effects of Porphyromonos gingivalis （P.gingivalis） lipopolysaccharide （LPS） on collagen phagocytosis of human periodontal ligament fibroblasts （hPDLF）. Methods Fluorescence localization and flow cytometry was used to test the collagen phygocytosis of hPDLF stimulated by the various concentration of LPS for 48 hours in vitro. Results The collagen phygocytosis of hPDLF was increased significantly by 0.1μg/mL LPS （P〈0.05）. Conclusion This study indicates that P.gingivalis LPS may enhance the degradation of collagen by stimulating the phagocytic activity of the hPDLF in periodontitis.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第4期339-341,共3页
West China Journal of Stomatology
关键词
牙龈卟啉单胞菌
脂多糖
牙周膜成纤维细胞
胶原
吞噬
Porphyromonos gingivalis； lipopolysaccharide；periodontal ligament fibroblasts；collagen
phagocytosis
