摘要
To study the lipolytic mechanism of EGCG,effects of EGCG(epigallocatechin-3-gallate)on lipolysis in rat primary adipocytes and mRNA expression of hormone-sensitive lipase(HSL),triacylglycerol hydrolase(TGH)were investigated through determination of glycerol release,triglyceride(TG)content and RT-PCR.The results showed that,EGCG of lower concentration(5 μmol/L)had no effects on glycerol release of mature adipocytes,while higher concentration could remarkable stimulate glycerol release compared with control group.Meanwhile,TG content in adipocytes of treated groups decreased in dose-dependent manners,proving that EGCG is an effective accelerant to lipolysis.Additionally,EGCG significantly down-regulated TGH and HSL mRNA expression,and the expression of TGH and HSL mRNA were very low when treated with 200 μmol/L EGCG.
To study the lipolytic mechanism of EGCG, effects of EGCG （epigallocatechin-3-gallate） on hpolysis in rat primary adipocytes and mRNA expression of hormone-sensitive lipase （HSL）, triacylglycerol hydrolase （TGH） were investigated through determination of glycerol release, triglyceride （TG） content and RT-PCR. The results showed that, EGCG of lower concentration （5 μmol/L） had no effects on glycerol release of mature adipocytes, while higher concentration could remarkable stimulate glycerol release compared with control group. Meanwhile, TG content in adipocytes of treated groups decreased in dose-dependent manners, proving that EGCG is an effective accelerant to lipolysis. Additionally, EGCG significantly down-regulated TGH and HSL mRNA expression, and the expression of TGH and HSL mRNA were very low when treated with 200 μmol/L EGCG.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2007年第4期767-772,共6页
ACTA ZOOLOGICA SINICA
基金
国家重点基础研究发展计划(973)项目~~
