摘要
作者将29例子宫内膜异位症（简称异位症）患者的异位和在位内膜进行离体组织孵育，应用放射免疫方法检测孵育液中泌乳素（PRL）含量；同时应用ABC免疫酶标记法确定异位和在位内膜分泌PRL的部位，结果异位和在位内膜离体组织经0～24h孵育，孵育液中PRL含量均显著增加（P＜0．05）；继续孵育至48h，PRL含量不再增加。但异位内膜分泌PRL的活性低于自身在位内膜。免疫组化PRL检测，异位和在位内膜分别有4和6例呈PRL阳性反应。组织学对照现察，内膜成熟上皮细胞数及前蜕膜细胞数越多者，其相应孵育液中PRL含量越高，免疫组化PRL阳性反应也越强。提示异位内膜PRL分泌特点与其自身在位内膜不完全相同，可能与病理环境有关。
Tissue incubation was used to determine the production of prolactin by ectopic and eutopic endometrium from 29 patients with endometriosis. The concentrations of prolactin in media were detected by radioimmunoassay (RIA ). Avidin-biotin immunoperoxidase method was used to demonstrate the presence of prolactin in ectopic and eutopic endometrium. Results: the levels of prolactin in media were significantly increased as ectopic and eutopic endometrial tissues incubated from 0 to 3 and to 24 h (P<0. 05 );yet,the contents of prolactin at 48 h in media were not increased. There was less secretory activity of prolactin in ectopic endometrium than in eutopic endometrium. Positive staining for prolactin was found in 4 ectopic endometria and 6 eutopic endometria. The histology showed that the more matured endometrial epithelial cells and predecidual cells,the higher concentrations of prolactin and the stronger prolactin staining. This suggested that secretive function of prolactin in ectopic endometrium was not the same as in eutopic endometrium,and it was related to pathologic environments.
关键词
子宫内膜异位
病理生理学
子宫内膜
催乳素
Endometriosis/physiopathol
Endometrium/secret
Radioimmunoassay
Prolactin
