摘要
目的 探讨甲氨基阿维菌素苯甲酸盐原药的致突变性及亚慢性毒性作用。方法 按照GB15670—1995《农药登记毒理学试验方法》进行Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠睾丸精母细胞染色体畸变试验及大鼠亚慢性毒性试验。结果 （1）50～200μg/皿剂量组的Ames试验结果为阴性。（2）染毒6.25～50.00 mg/kg剂量组的微核率与阴性对照组比较差异无统计学意义（P〉0.05）,甲氨基阿维菌素苯甲酸盐原药不引起小鼠骨髓多染红细胞染色体断裂或整条染色体丢失。（3）染毒12.5～50.0 mg/kg剂量组的小鼠睾丸初级精母细胞染色体畸变率未见增加。（4）亚慢性毒性试验高剂量组部分动物在染毒后期出现全身震颤、精神不振、被毛蓬松、体重增长缓慢等中毒症状。实验结束时,高剂量组雌鼠淋巴细胞比例降低、中间细胞比例增高,肝脏脏器系数明显增高;雄鼠肾脏和脑的脏器系数增高。中、高剂量组雄鼠淋巴细胞比例降低、中间细胞比例增高,心脏脏器系数增高。结论 甲氨基阿维菌素苯甲酸盐原药无明显致突变作用。该药可引起动物出现神经系统症状,影响动物的生长发育及免疫功能。本实验提示,SD大鼠口服甲氨基阿维菌素苯甲酸盐原药90 d的最大无作用剂量为0.89 mg.kg-1.d-1。
Objective To explore the mutagenicity and subehronic toxicity of methylaminoemameetin benzoate, Method The Ames assay, mouse bone marrow cell miemnueleus test, mouse testicle cell chromosome aberration test and subehrunie toxicity test in rats were conducted according to National Standards of ＂Toxicological Methods of Pesticides for Registration＂ （GB15670-1995 of PRC）. Result 50 - 200 μg/plate of emameetin benzoate did not induce positive mutations of strains TA97, TA98, TA100, TA102 in Ames test. Mouse bone marrow cell miemnueleus test （6.25 - 50.00 mg/kg） showed that the miemnueleus rate in each dosage group significantly increased compared with control group （ P 〉 0.05）, and it also so did in mouse testicle cell chromosome aberration test （ 12.5 - 50.0 mg/ kg ）. Subehronie toxicity test showed that at highest dosage （8.0 mg·kg^-1＂ d^-1）, part of the administrated rats presented some clinical symptoms such as general fremitus, poor spirit, blowzy fur, slow growth of body weight during 11 to 13 weeks after experiment; at the end of experiment, there were some decrease of lymphocyte proportion, increase of intermediate cells （MID） proportion and obvious rise of liver coefficiente in female rats, but there were some increases of kidney and brain coefficientes in male rats. While in middle and high dosage groups, the decrease of lymphocyte proportion, increase of intermediate cells （MID） proportion and obvious rise of heart coefficiente only could be seen in male rats. There was no drug-related effects in rats at the dose level of 0.89 mg· kg^-1· d^-1. Conclusion The emameetin benzoate did not show any obvious mutagenieity, but it could induce nervous symptoms, interfere with body-weight growth and immunity function. The maximal non-effect dose of emameetin benzoate under oral administration for 90 days in SD rats should be 0.89mg· kg^-1· d^-1 acceding to this experiment.
出处
《中国工业医学杂志》
CAS
北大核心
2007年第4期221-224,共4页
Chinese Journal of Industrial Medicine
