摘要
目的:建立胆南星中猪去氧胆酸含量测定方法,为其质量控制提供方法。方法:用HPLC-蒸发光散射检测测定样品的猪去氧胆酸含量。色谱和检测条件:Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),柱温35℃;流动相甲醇-水-冰醋酸(75∶25∶0.01),流速1 mL.min-1;漂移管温度85℃,空气流速3.0 L.min-1。结果:猪去氧胆酸进样量在2.4～24μg,进样量和峰面积的对数呈线性(r=0.999 8)。平均加样回收率为97.1%,RSD 3.0%(n=5)。结论:方法简便,测定准确,且重复性好,可用于胆南星的质量控制。
Objective： To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema. Method： The methanol extracts were separated on a Diamonsil ODS column eluted at 35 ℃ with a mixture of methanol-water-glacial acetic acid （75：25：0.01） at flow-rate 1.0 mL · min^-1. The drift-tube temperature of EKSD was 85 ℃ ,and the flow rate of air was 3.0 L · min^-1. Result： Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear （r =0.999 8） over the range of 2.4-24 μg. The average recovery was 97.1% （RSD 2.9%, n =5）. Conclusion： The method could be used for quality control of Bile Arisaema.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第16期1634-1636,共3页
China Journal of Chinese Materia Medica
