摘要
以自制的几丁质作载体，戊二醛作交联剂，用吸附交联法对菊粉酶进行了固定化研究。优化了固定化反应条件，在最适条件下，菊粉酶活力最大收率为32％。固定化菊粉酶的最适温度为60～65℃，比游离酶提高5℃。最适pH保持不变。固定化酶的热稳定性和对酸碱稳定性都有较明显的改善，将固定化酶装柱进行菊芋汁连续水解试验，操作半衰期达22d。
Inulinase was immobilized on Chitin,with glutaraldeyde as crosslinking agent. Theconditions of immobilization were sdudied systematically,and under the optimal conditions,the enzyme activity recorery of the immobilized inulinase was 33%. The optimal temperatureof the immobilized enzyme was at the range of 60~ 65℃ which was higher than that of thesoluble inulinase by 5℃. The optimal pH was not changed by immobilization. Thermal stabil-ity of the inulinase was improved by immobilizing treatment. so did the pH stability. Underthe condition of 6O℃,the half-life of the immobilized inulinase was 22 days.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1997年第3期80-84,共5页
Journal of Hebei Agricultural University
基金
河北省自然科学基金
关键词
固定化酶
菊粉酶
几丁质
Immobilized inulinase
Inulinase
Chitin
