摘要
目的:观察肾精亏虚对雄性小鼠及其雄性仔鼠生育力的影响。方法:将30只6周龄雄性昆明小鼠随机均分为正常对照组、模型组和补肾组3组。模型组和补肾组采用房劳加惊恐的复合伤肾法制造肾精亏虚模型。补肾组每天给予0.16ml/10g体重的补肾填精方浓缩液灌胃。正常对照组及模型组予等量生理盐水灌胃,造模并给药21d。从造模结束次日起,所有小鼠与正常动情期雌鼠配对饲养5d后检测其精子密度及活动率。取出雌鼠待其分娩,分别计算各组配对雌鼠妊娠率和每胎生产数(仔鼠总数/妊娠并分娩的雌鼠只数)以评估父代小鼠生育力。雄性仔鼠饲养至6周龄时每组随机取出10只检测其精子密度及活动率。结果:模型组每胎生产数[(7.00±1.73)只]明显比正常对照组[(9.43±1.27)只]和补肾组[(8.80±1.10)只]低(P<0.05);模型组小鼠精子密度[(9.70±1.45)×106/ml]及活动率[(66.72±10.12)%]均明显低于正常对照组[(14.08±1.15)×106/ml,(81.75±3.56)%]和补肾组[(12.20±1.55)×106/ml,(78.55±4.38)%],P<0.01,而正常对照组与补肾组之间则没有差异(P>0.05);模型组仔鼠精子密度[(10.1±1.79)×106/ml]及活动率[(71.86±7.48)%]均低于正常对照组[(15.30±1.83)×106/ml,(79.86±5.68)%]和补肾组仔鼠[(14.20±2.21)×106/ml,(81.92±2.51)%],P<0.05,正常对照组与补肾组仔鼠之间没有差异(P>0.05)。结论:"惊恐、房劳"可以明显降低小鼠生育力,其仔鼠生育力亦可受累。补肾法可以阻断这种损伤的发生。
Objective： To investigate the effects of kidney-jing deficiency on the fertility of male mice and their male offspring. Methods ： Thirty 6-week-old Kunming male mice and 300 female ones were randomly allocated to a blank control group, a model group and a kidney-tonifying group. The model and the kidney-tonifying groups were stressed by fear plus excessive sex to establish a kidney- jing deficiency model, and meanwhile the latter were given concentrated solution of Kidney-tonifying Recipe intragastrically at the dose of 0.16 ml / 10 g. The control and the model groups were treated with physiological saline at the same dose for 21 days. Then all the male mice were mated with the healthy estrous females for 5 days. The sperm density and motility of each group of the male mice were examined, and their fertility was assessed by comparison of the pregnancy rate and the number of baby mice at each birth among their female mates. And the sperm density and motility of the male offspring were detected at 6 weeks. Results ： The average number of baby mice at each birth in the model group was （7.00 ± 1.73 ）, significantly smaller than those in the control （9.43 ± 1.27） and the kidney-tonifying group （ 8.80 ± 1.10） （ P 〈 0.05 ）. The sperm density and motility of the model mice were （9.70 ± 1.15） × 10^6/ml and （66.72 ± 10.12） %, lower than those of the control （[14.08 ± 1.15] × 10^6/ml and [81.75 ± 3.56] %）, andthe kidney-tonifying group （ [ 12.20 ± 1.55 ] × 10^6/ml and [ 78.55 ± 4.38 ] % ） （P 〈 0.05）. There was no significant difference between the latter two groups （ P 〉 0.05 ）. The sperm density and motility of the offspring of the model mice were （ 10. 10 ± 1.79 ） × 10^6/ml and （71.86 ± 7.48） %, lower than those of the control （ [ 15.30 ± 1.83 ] × 10^6/ml and [ 79.86 ± 5.68 ] % ）, and the kidney-tonifying group （ [ 14.20 ± 2.21 ] × 10^6/ml and [ 81.92 ± 2.51 ] % ） （ P 〈 0.05 ）, with no significant difference between the latter t
出处
《中华男科学杂志》
CAS
CSCD
2007年第8期754-757,共4页
National Journal of Andrology
基金
湖北省自然科学基金(编号2004ABA184)
关键词
肾精亏虚
房劳
惊恐
生育力
小鼠
kidney-jing deficiency
excessive sex
fear
fertility
mouse
