摘要
目的研究重症肌无力(MG)患者外周血多群调节性 T 细胞的水平及其 B 细胞表达 B细胞激活因子受体(B cell-activating factor receptor,BAFF-R)的情况。方法应用四色流式细胞仪检测61例 MG 患者与23名健康对照外周血调节性 T 细胞(CD_4^+ CD_(25)^(high)Foxp3^+、CD_8^+CD_(28)^-、CD_8^+CD_(122)^+)以及 CD_(19)^+BAFF-R^+细胞的百分率。结果 MG 组与健康对照组外周血 CD_4^+CD_(25)^(high)Foxp3^+T 细胞的百分率分别为32.12%±16.12%与65.15%±14.72%,MG 组该群调节性 T 细胞的水平明显低于健康对照组(P<0.01);两组 CD_8^+CD_(28)^-及 CD_8^+CD_(122)^+T 细胞的水平差异无统计学意义。此外,MG 组外周血CD_(19)^+BAFF-R^+细胞的水平(10.57%±5.59%)显著高于健康对照组(5.38%±3.87%,P<0.01)。大剂量激素或大剂量激素加丙种球蛋白治疗后短期内可使 MG 组外周血 CD_4^+CD_(25)^(high)Foxp3^+调节性 T 细胞的百分率增加(P<0.05)。结论 MG 患者 Foxp3^+的 CD_4^+CD_(25)^(high)调节性 T 细胞的减少提示 MG 患者存在免疫和耐受的失衡,显示了 T 细胞的自身免疫性。在 B 细胞方面,MG 患者外周血 CD_(19)^+B 细胞上 BAFF-R 表达增高,提示其体内 B 细胞已处于易激活状态。
Objectives To investigate the levels of circulating regulatory T cells and B cellactivating factor receptor （BAFF-R） on B cells from patients with myasthenia gravis （MG）. Methods The levels of circulating regulatory T cells（ CD4^＋ CD25^high Foxp3^＋ , CD8^＋ CD28^-, CD8^＋ CD122^＋ ） and CD19^＋ BAFF-R^＋ cells in 61 MG patients and 23 healthy controls were tested with flow cytometry. Results The percentages of the circulating CD4^＋CD25^high Foxp3^＋ T cells （65.15% ± 14. 72% ） in MG patients were significantly decreased compared with healthy controls（32. 12% ± 16. 12% ,P 〈0. 01 ）. The expression of BAFF-R on CD19^＋B cells in MG patients （ 10. 57%±5.59% ） was higher than that in healthy controls （ 5.38%± 3. 87%, P 〈 0. 01 ）. There was no significant difference in the subpopulation of CD8^＋ CD28^- and CD8^＋ CD122^＋T cells between MG patients and healthy controls. Treatment of high-dose corticosteroid with or without additional intravenous IgG elevated the percentages of CD4^＋ CD25^high Foxp3^＋ T cells in 20 MG patients （ P 〈 0. 05 ）. Conclusions These results reveal that decrease of circulating CD4^＋ CD25^high Foxp3^＋ regulatory T cells in MG patients might be related to the pathogenesis of MG. An elevated BAFF-R on CD19^＋ B cells demonstrates that B cells are more active in MG patients.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2007年第8期512-515,共4页
Chinese Journal of Neurology
基金
国家自然科学基金(30571727)
