摘要
采用紫外分光光度法、ＴＢＡ法、琼脂糖凝胶电泳法检测维生素Ｃ或Ｈ２Ｏ２体系对ＤＮＡ的作用，发现ＤＮＡ在２６０ｎｍ处吸光值下降，检测到ＤＮＡ的脱氧核糖损伤，观察到ＤＮＡ链降解．痕量的过渡金属离子Ｆｅ２＋等能加速上述反应的进行，各种ＨＯ·清除剂可抑制上述反应．表明在上述两类反应体系中产生了ＨＯ·，是ＨＯ·破坏ＤＮＡ的碱基和脱氧核糖。
DNA damage by oxygen radical was studied in the systems containing ascorbate or H 2O 2, using ultraviolet-spectrometer, TBA reaction and agarose gel electrophoresis. The absorption in 260nm of DNA decreased. Deoxyribose damage was found in TBA reaction. DNA backbone cleavage was observed in agrose gel electrophoresis. Trace metal ion such as Fe 2+ and so on could accelerate the reactions mentioned above. In all case DNA damage was prevented by a series of HO · scarvages. The role of HO · induced by the above two systems in the DNA damage including base, deoxyribose and backbone degradation was discussed.
出处
《武汉大学学报（自然科学版）》
CSCD
1997年第2期238-242,共5页
Journal of Wuhan University(Natural Science Edition)
基金
国家教委留学回国人员科研基金
湖北省自然科学基金
