摘要
通过采用Fullaondo(1999)研究的特异引物检测方法,对来自英国和比利时的两个马铃薯金线虫(Globodera rostochiensis)种群和英国的一个马铃薯白线虫(G.pallida)种群的DNA进行扩增,其产物以大肠杆菌(Eschetichia coli)为受体菌,经过连接、转化和克隆纯化,筛选获得阳性克隆。经PCR鉴定和测序,英国和比利时的马铃薯金线虫种群的特异性谱带分别是357bp和350bp,而英国的马铃薯白线虫的谱带是782bp,与Fullaondo等人研究报道结果基本相符,从而鉴别出来自不同地理种群的马铃薯金线虫和白线虫。
The study based on the specific primer method of Fullaondo(1999),2 golden cyst nematode population’s DNA origin from England and Belgium and 1 white cyst nematode population from England were amplified by PCR. The products target to bacteria Eschetichia coli by linking,transforming and culturing in the agar,one-cell isolate to be pury clone,a positive-clone isolate.The isolate liquit making test by PCR before sequence getting.The result showed the specific band for golden cyst nematode were 357bp and 350bp independently,while analysis that of white cyst nematode was 782bp.Compared to Fullaondo’ s study,both results were similar.This mehtod could correctly distinguish different geographic populations of golden cyst nematode and white cyst nematode.
出处
《植物检疫》
2007年第4期198-200,共3页
Plant Quarantine
